Galactosylation and glucosylation by use of β-galactosidase
1992; Elsevier BV; Volume: 33; Issue: 2 Linguagem: Inglês
10.1016/0040-4039(92)88050-f
ISSN1873-3581
AutoresBernd Sauerbrei, Joachim Thiem,
Tópico(s)Enzyme Production and Characterization
ResumoThe transglycosylation activity of β-galactosidase derived from Aspergillus oryzae and Escherichia coli, respectively, was examined in reaction systems containing up to 50% acetonitrile. Starting with ortho-nitrophenyl β-galactoside (1), which functions both as donor and as acceptor. β-Gal(1-6)]β-Gal-PhNO2-o (2) and β-Gal(1-3)β-Gal-PhNO2-o (3) were obtained. Under similar conditions the enzyme from A. oryzae converts para-nitrophenyl β-glucoside (5) to β-Glc(1-2)β-Glc-PhNO2-p (6) and α-Glc(1-4)β-Glc-PhNO2-p (7). Incubation of 1 and L-serine in the presence of the A. oryzae β-galactosidase leads to β-Gal-L-Ser (4).
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