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Mast cells in human dental pulp

1984; Elsevier BV; Volume: 10; Issue: 6 Linguagem: Catalão

10.1016/s0099-2399(84)80057-6

1878-3554

Ali Farnoush,

Fluorine in Organic Chemistry

Controversy exists regarding the presence of mast cells in the dental pulp. As mast cells are present in other soft tissues throughout the body, their reported absence in the dental pulp seems puzzling and anomalous. Dental pulps from 10 caries-free permanent and 10 carious primary teeth were expamined for the presence of mast cells. The pulps were removed by splitting the teeth, frozen-sectioned at 5 μm, and stained for mast cells utilizing a technique which is proven to be highly selective for identification of mast cells in various tissues. Additional sections from inflamed and noninflamed pulps were stained with hematoxylin and eosin to assess the inflammatory response. The results indicated that mast cells were present in noninflamed as well as inflamed pulps. Mast cells from the inflamed pulps showed signs of degranulation with granules appearing outside the cell membrane. Controversy exists regarding the presence of mast cells in the dental pulp. As mast cells are present in other soft tissues throughout the body, their reported absence in the dental pulp seems puzzling and anomalous. Dental pulps from 10 caries-free permanent and 10 carious primary teeth were expamined for the presence of mast cells. The pulps were removed by splitting the teeth, frozen-sectioned at 5 μm, and stained for mast cells utilizing a technique which is proven to be highly selective for identification of mast cells in various tissues. Additional sections from inflamed and noninflamed pulps were stained with hematoxylin and eosin to assess the inflammatory response. The results indicated that mast cells were present in noninflamed as well as inflamed pulps. Mast cells from the inflamed pulps showed signs of degranulation with granules appearing outside the cell membrane. Existen controverias sobre la presencia de mastocitos en pulpas dentales. Como los mastocitos estan presentes en otros tejidos blandos del organismo su ausencia en la pulpa dental resulta curiosa y anormal. Fueron observadas las pulpas dentales de 10 dientes permanentes libres de caries y de 10 dientes primarios con caries. Las pulpas fueron removidas por corte de los dientes, congeladas, seccionadas a 5 micrones y coloreados utilizando una técnica que probó ser altamente selectiva para la identificación de mastocitos en tejidos varios. Otras secciones de pulpas inflamadas y no inflamadas fueron coloreadas con hematoxilina-eosina para evaluar la respuesta inflamatoria. Los resultados indicaron que los mastocitos estan presentes en pulpas tanto inflamadas como no inflamadas. Mastocitos proveniende pulpas inflamadas mostraron signos de degranulación con gránulos por fuera de la membrana celular. Existen controverias sobre la presencia de mastocitos en pulpas dentales. Como los mastocitos estan presentes en otros tejidos blandos del organismo su ausencia en la pulpa dental resulta curiosa y anormal. Fueron observadas las pulpas dentales de 10 dientes permanentes libres de caries y de 10 dientes primarios con caries. Las pulpas fueron removidas por corte de los dientes, congeladas, seccionadas a 5 micrones y coloreados utilizando una técnica que probó ser altamente selectiva para la identificación de mastocitos en tejidos varios. Otras secciones de pulpas inflamadas y no inflamadas fueron coloreadas con hematoxilina-eosina para evaluar la respuesta inflamatoria. Los resultados indicaron que los mastocitos estan presentes en pulpas tanto inflamadas como no inflamadas. Mastocitos proveniende pulpas inflamadas mostraron signos de degranulación con gránulos por fuera de la membrana celular.