An upstream repressor element plays a role in Igf2 imprinting
2001; Springer Nature; Volume: 20; Issue: 13 Linguagem: Inglês
10.1093/emboj/20.13.3518
ISSN1460-2075
Autores Tópico(s)Gestational Trophoblastic Disease Studies
ResumoArticle2 July 2001free access An upstream repressor element plays a role in Igf2 imprinting Sharon Eden Sharon Eden Department of Cellular Biochemistry, Hebrew University, Ein Kerem, Jerusalem, 91120 Israel Search for more papers by this author Miguel Constancia Miguel Constancia Programme in Developmental Genetics, The Babraham Institute, Cambridge, CB2 4AT UK Search for more papers by this author Tamar Hashimshony Tamar Hashimshony Department of Cellular Biochemistry, Hebrew University, Ein Kerem, Jerusalem, 91120 Israel Search for more papers by this author Wendy Dean Wendy Dean Programme in Developmental Genetics, The Babraham Institute, Cambridge, CB2 4AT UK Search for more papers by this author Beth Goldstein Beth Goldstein Department of Cellular Biochemistry, Hebrew University, Ein Kerem, Jerusalem, 91120 Israel Search for more papers by this author Alfred C. Johnson Alfred C. Johnson Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA Search for more papers by this author Ilana Keshet Ilana Keshet Department of Cellular Biochemistry, Hebrew University, Ein Kerem, Jerusalem, 91120 Israel Search for more papers by this author Wolf Reik Wolf Reik Programme in Developmental Genetics, The Babraham Institute, Cambridge, CB2 4AT UK Search for more papers by this author Howard Cedar Corresponding Author Howard Cedar Department of Cellular Biochemistry, Hebrew University, Ein Kerem, Jerusalem, 91120 Israel Search for more papers by this author Sharon Eden Sharon Eden Department of Cellular Biochemistry, Hebrew University, Ein Kerem, Jerusalem, 91120 Israel Search for more papers by this author Miguel Constancia Miguel Constancia Programme in Developmental Genetics, The Babraham Institute, Cambridge, CB2 4AT UK Search for more papers by this author Tamar Hashimshony Tamar Hashimshony Department of Cellular Biochemistry, Hebrew University, Ein Kerem, Jerusalem, 91120 Israel Search for more papers by this author Wendy Dean Wendy Dean Programme in Developmental Genetics, The Babraham Institute, Cambridge, CB2 4AT UK Search for more papers by this author Beth Goldstein Beth Goldstein Department of Cellular Biochemistry, Hebrew University, Ein Kerem, Jerusalem, 91120 Israel Search for more papers by this author Alfred C. Johnson Alfred C. Johnson Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA Search for more papers by this author Ilana Keshet Ilana Keshet Department of Cellular Biochemistry, Hebrew University, Ein Kerem, Jerusalem, 91120 Israel Search for more papers by this author Wolf Reik Wolf Reik Programme in Developmental Genetics, The Babraham Institute, Cambridge, CB2 4AT UK Search for more papers by this author Howard Cedar Corresponding Author Howard Cedar Department of Cellular Biochemistry, Hebrew University, Ein Kerem, Jerusalem, 91120 Israel Search for more papers by this author Author Information Sharon Eden1, Miguel Constancia2, Tamar Hashimshony1, Wendy Dean2, Beth Goldstein1, Alfred C. Johnson3, Ilana Keshet1, Wolf Reik2 and Howard Cedar 1 1Department of Cellular Biochemistry, Hebrew University, Ein Kerem, Jerusalem, 91120 Israel 2Programme in Developmental Genetics, The Babraham Institute, Cambridge, CB2 4AT UK 3Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA *Corresponding author. E-mail: [email protected] The EMBO Journal (2001)20:3518-3525https://doi.org/10.1093/emboj/20.13.3518 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The imprinted Igf2 gene is associated with a small upstream region that is differentially methylated on the active paternal allele. We have identified a repressor element within this sequence and shown that repression is probably mediated through a trans- acting factor, GCF2. DNA methylation of this site abrogates both protein binding and repressor activity. Targeting experiments demonstrate that this element plays a role in the repression of the maternal Igf2 gene in vivo. Introduction The DNA methylation pattern of the animal genome is established during development through a process of de novo methylation and demethylation which is directed by cis-acting elements (Razin, 1998). In contrast to most of the genome where both alleles are methylated to an equal extent, genomically imprinted domains are characterized by the inclusion of small regions that are differentially methylated on the two alleles (DMRs) (Razin and Cedar, 1994; Constancia et al., 1998). From the developmental point of view, these DMRs fall into two distinct categories. In one type, the differential methylation pattern is established in the gametes and then maintained throughout embryogenesis. These modifications serve to mark the two parental alleles so that they can be identified in somatic cells as being of either maternal or paternal origin. Examples include previously characterized sequences upstream of the H19 gene (Bartolomei et al., 1993) or within the Igf2r intron in the mouse (Stoger et al., 1993). A second class of differential methylation is established post-zygotically (Brandeis et al., 1993; Feil et al., 1994). Since these methyl groups are not put on during gametogenesis, the resulting allele-specific pattern must be generated using structural cues already present on the parental alleles. Although it is generally assumed that these DMRs are involved in the local control of allele-specific gene expression, little is known about their mechanisms of action. A good model system for studying this phenomenon is the mouse Igf2 domain, which is associated with three distinct post-zygotically established DMRs located at different positions in the vicinity of this gene (Moore et al., 1997). Two of these regions are methylated on the active paternal allele, a pattern that is contrary to the accepted dogma that methylation mediates repression in cis. One model for explaining this anomalous behavior proposes that these regions contain repressor elements that interact with trans-acting factors whose binding can be blocked by DNA methylation (Sasaki et al., 1992; Brandeis et al., 1993; Feil et al., 1995). According to this idea, the maternal unmethylated Igf2 allele is transcriptionally inactive because of these cis-acting repressor elements, while the paternal allele adopts an active expression profile by virtue of DNA modification which prevents repressor function. In keeping with this, it has been shown that both the maternal and paternal copies of the Igf2 gene are transcriptionally inactive when the DMRs are unmethylated on both alleles, as occurs in methylase−/− embryos (Li et al., 1993). Using gene targeting, it has been demonstrated that a 5 kb region upstream to the Igf2 gene contains repressor sequences (Constancia et al., 2000), but the elements themselves were not mapped and the mechanism of repression not studied. Here, we have used a transfection assay to show that a differentially methylated region (DMR1) within this same stretch of DNA serves as a transcriptional repressor and that its function can be abrogated by DNA methylation. This mechanism is actually mediated by a small element within the DMR, which probably interacts with the previously characterized repressor protein GCF2 (Kageyama and Pastan, 1989; Reed et al., 1998). Using sequence-specific gene targeting in mice, we have demonstrated that this site is required for repressing transcription from the maternal Igf2 allele, indicating that this regulatory mechanism is used in vivo as part of the imprinting process. Results The Igf2 upstream region is differentially methylated Previous studies have shown that the region upstream of Igf2 in the mouse is differentially methylated (DMR1) in a number of tissues. One of the difficulties in assaying methylation in this region stems from the fact that it contains four closely spaced HpaII sites, which cannot be distinguished easily on Southern blots (Sasaki et al., 1992; Brandeis et al., 1993; Feil et al., 1994). In order to overcome this problem, we utilized the methyl-sensitive enzyme NgoMI, which recognizes HpaII site 4 exclusively (Figure 1A). Mice of a normal Mus m. domesticus genetic background, but with the distal portion of chromosome 7 derived from M.spretus (SD7), were crossed with standard M.m.domesticus mice (Forne et al., 1997) and DNA from the offspring analyzed for DNA methylation. In these animals, it was possible to distinguish between the parental alleles by taking advantage of a spretus-specific polymorphic DraI restriction site located within DMR1. As shown in Figure 1B, regardless of whether the SD7 mouse serves as the mother or the father, the NgoMI site is methylated (∼85%) when derived paternally, but unmethylated when inherited from the mother. These results thus show that there are indeed clearcut differences in methylation between the maternal and paternal alleles in this Igf2 upstream region. Figure 1.Igf2 allelic methylation and histone acetylation patterns. (A) Map of the mouse Igf2 gene indicating the promoters (P0–P3), the differentially methylated (DMR1) region including HpaII sites 2–5, restriction sites for E (EcoRI), X (XbaI), N (NgoMI; NgoMIV), D (DraI; only present on the M.spretus allele), the EcoRI–XbaI probe and the position of the PCR fragment used in the immunoprecipitation experiments. (B) Igf2 differential methylation at site 4 was measured by Southern blot analysis of kidney genomic DNA from SD7 heterozygous mice using the restriction enzymes shown in each lane. After digestion with NgoMI, both the domesticus allele (3 kb) and the spretus allele (1.3 kb) yield a 1.25 kb band. Similar results were obtained using DNA from liver but, in this case, the paternal allele was only 70% methylated. (C) The acetylation state of nucleosomes in DMR1 was determined by immunoprecipitation of mononucleosomes from SD7 heterozygous mice using anti-acetylated H4 histone. DNA from input and bound fractions was extracted and 1 or 3 μl subjected to quantitative radiolabeled PCR with primers specific for sequences from the κ-light chain, β-actin and Igf2 upstream regions (left panel). The Igf2 PCR product was digested with HpaII to identify a polymorphic site on this fragment, present only on the spretus (paternal) allele, analyzed by gel electrophoresis (right panel) and the results quantitated using a phosphoimager. The ratio between the maternal and paternal alleles in the bound fraction was found to be 5.5-fold more than that measured for the input DNA. In a separate experiment, we also determined that the PCR product obtained from total DNA is produced equally from the two alleles (data not shown). Download figure Download PowerPoint Previous studies have shown that both Igf2 alleles in the nucleus are equally sensitive to DNase I in the promoter region, suggesting that they have similar overall structures (Sasaki et al., 1992; Feil et al., 1995). However, since it is known that DNA methylation can have a profound effect on DNase I sensitivity and chromatin structure in general (Keshet et al., 1986), we asked whether DMR1 may be characterized by local allele-specific differences in structure. It was demonstrated recently, for example, that DNA methylation itself can affect chromatin by mediating the deacetylation of local histone proteins (Eden et al., 1998; P.L.Jones et al., 1998; Nan et al., 1998). In order to determine whether this might be the case for DMR1, we immunoprecipitated nucleosomes rich in acetylated histone H4 from primary fibroblast nuclei of musculus/SD7 F1 mice and then used PCR to assay the representation of several different sequences in this fraction. As shown in Figure 1C, the presence of acetylated histones correlates well with gene activity in control experiments. The constitutive β-actin gene sequence, for example, is highly enriched in this acetylated nucleosome fraction, whereas the unexpressed κ gene sequence is under-represented. As demonstrated in Figure 1C, DMR1 is relatively rich in acetylated histones. However, when the PCR product was cut with HpaII, which recognizes a polymorphic site that distinguished between the two alleles, one can see immediately that the unmethylated maternal allele is more acetylated than its paternal counterpart (5.5-fold) even though both alleles are equally represented in the starting material before immunoprecipitation. These findings thus suggest that this upstream region may be packaged into a relatively more open chromatin structure when present on the maternal allele, consistent with the idea that this domain is available for interacting with repressor factors, while the paternal allele is relatively closed. Thus, this region appears to carry a chromatin structural imprint. DMR1 contains a functional repressor element Since it is the inactive maternal Igf2 allele that is characterized by undermethylation of upstream sequences, it has been suggested that this DMR may harbor a methyl-sensitive repressor element. In confirmation of this prediction, a simple sequence analysis of this region indeed revealed the presence of a well known repressor element that had been shown previously to interact with the protein, GCF (Johnson et al., 1992). Furthermore, this element co-maps precisely with the same HpaII site (site 4) that we have shown to be differentially methylated in vivo (see Figure 1B). In order to determine directly whether sequences within the DMR1 region actually play a role in the control of Igf2 expression, we studied its effect on transcription by employing a transient transfection assay in human kidney cells (293 cells). To this end, we made a test construct with a CAT reporter gene driven by the herpes thymidine kinase (TK) minimal promoter and examined the influence of DNA inserts from the DMR1 region. The presence of a full 2.2 kb Igf2 upstream region containing all of the differentially methylated HpaII sites (2–5) caused a 4- to 5-fold inhibition of transcription in this system (Figure 2B). Furthermore, this repression was relieved when the plasmid was modified using HpaII methylase. It should be noted that in the absence of repressor sequences, the TK CAT vector itself, which does not contain any HpaII sites in its minimal promoter, is slightly inhibited by this methylation. Despite this background, methylation of the repressor element apparently overcomes inhibition and brings about an increase in transcription. Figure 2.DMR1 contains a methylation-sensitive repressor element. Transient transfection into 293 human kidney cells was used to measure repressor activity on a pTK-CAT reporter construct. (A) Map showing the different constructs used in the transient transfection experiments. (B) CAT assays of reporter constructs. The results are shown in graphic form relative to the basic TK-CAT vector. These values were determined by scanning autoradiograms. For each lane, the particular construct used is indicated by a modular diagram made up of fragments from DMR1, the TK promoter and the CAT reporter gene. The last construct (disruption) is the same as the 340 bp fragment but contains a loxP insertion (see text). Constructs were either unmethylated (−) or in vitro methylated by MHpaII (+). The HpaII sites (2–5) located within each fragment are indicated in the diagram. Most of these experiments were repeated 4–5 times and the results shown represent average values (± SD). Download figure Download PowerPoint These initial experiments indicated that the sequences responsible for repression probably map to the region containing HpaII sites 3 and 4, since insertion of a smaller 340 bp fragment into the test vector brought about a similar degree of repression, which could also be relieved by DNA methylation (Figure 2B). In order to pinpoint further the repressor element itself, we generated a number of reporter constructs carrying the TK CAT gene together with small upstream sequences derived from within a 680 bp fragment (see Figure 2A). These experiments demonstrated conclusively that only fragments containing HpaII site 4 are capable of mediating the repressor effect (data not shown), and a minimal 150 bp fragment was found to be sufficient for both repression and methylation reversal (Figure 2B). While these data show that sequences around HpaII site 4 are sufficient for methyl-sensitive repression in the transfection assay, they do not prove that this element is necessary for the transcriptional repression mediated by DMR1. In order to address this question, we compared the full 2.2 kb DMR1 fragment with a similar sequence that lacks a 420 bp region containing HpaII sites 3 and 4. As shown in Figure 2B, this deletion significantly lowers the level of repression. More importantly, DNA methylation now brings about a slight inhibition of transcription, rather than the enhancement normally seen when the repressor element is present. Similarly, we specifically disrupted site 4 itself by inserting a loxP element in the critical sequence, and this was sufficient to eliminate the repression usually associated with the 340 bp fragment (Figure 2B). Protein interaction is required for repression These results suggested that trans-acting repressor factors in the nucleus interact with sequence elements within the Igf2 upstream region, and that DNA methylation prevents this binding. To test this possibility directly, we carried out co-transfection competition experiments using the CAT expression vector together with an excess of a second plasmid containing only 680 bp of DMR1. As seen in Figure 3A, the addition of non-methylated competitor DNA completely reverses the repression profile, while methylated DNA has no effect. This clearly indicates that repressor proteins bind to unmethylated, but not methylated DNA and, since this plasmid was only modified at HpaII sites, it must be these particular sequences that are involved in repression. Figure 3.Repression is mediated by protein interactions with DMR1. (A) Co-transfection competition experiments were carried out using a reporter construct containing a 680 bp fragment from DMR1 (see Figure 2A) and competitor plasmid DNA containing only the 680 bp fragment (10- or 20-fold excess in methylated or unmethylated form), and the results (average ± SD) are shown as in Figure 2. The pTk-CAT vector was unaffected by the addition of either unmethylated or methylated competitor DNA (data not shown). (B) Gel shift analysis of a 32P-end-labeled Igf2 upstream oligonucleotide using 293 cell nuclear extract. Competition (left) was carried out using a 45-fold excess of non-radioactive wild-type (wt), methylated or disrupted oligonucleotides (see Materials and methods). Gel shift analysis was also carried out (middle) with the same extract treated with two different concentrations of GCF2 antibody to bring about immunodepletion. In a separate experiment, we removed the antibody complex from the Sepharose beads and confirmed by western analysis that it contained GCF2. A mock experiment in which the extract was passed over protein A–Sepharose beads without antibody is also shown. Extract or pure recombinant GCF2 protein was reacted (right) with either the labeled Igf2 upstream oligonucleotide (I) or an oligonucleotide containing the EGFR recognition sequence (E). A small amount of immunodepleted extract was added to the pure protein in order to stabilize it and enhance binding. Download figure Download PowerPoint These transfection experiments clearly suggest that HpaII site 4 contains a sequence element that mediates gene repression through interaction with proteins present in the nucleus. To test this hypothesis directly, we carried out gel retardation experiments in vitro (Figure 3B). An end-labeled 20 bp fragment containing HpaII site 4 was clearly shifted when exposed to nuclear extract from human kidney cells (293 cells). This binding activity apparently is sequence specific, since it could be competed out with unlabeled oligonucleotide, but not with identical fragments either mutated or containing a small disruption at the HpaII site. Furthermore, binding was not competed out by a methylated oligonucleotide, consistent with the observation that methylation prevents repression at this site. Since this binding site contains a GCF motif (Kageyama and Pastan, 1989), we wanted to test the possibility that it is this factor that is responsible for the band shift. GCF, initially characterized as a presumed transcriptional repressor, recently has been shown to be derived from an artifactual cDNA fusion product, which, however, carries the DNA-binding domain of an authentic endogenous protein, GCF2 (Reed et al., 1998; Takimoto et al., 1999). Evidence that GCF2 binds the Igf2 repressor element was obtained by immunodepleting this protein from the nuclear extract using a specific antibody, and this indeed resulted in elimination of the band shift. Furthermore, purified recombinant GCF2 protein itself produces a band shift similar to that seen with the full extract (Figure 3B), and almost identical to a band generated through interaction with a prototypic GCF2 element from the EGFR gene (Reed et al., 1998). These experiments strongly suggest that GCF2 interacts with the Igf2 upstream region and is probably responsible for the repression observed in transfection assays. The upstream repressor element influences Igf2 expression in vivo In order to determine whether the repressor element upstream of the Igf2 gene actually plays a role in the control of imprinted expression in vivo, we designed an experiment to disrupt this element by DNA targeting of the endogenous gene in the mouse. To this end, we generated a vector containing 5 kb of sequence upstream of the Igf2 gene and then inserted a loxP-flanked Neor gene into the GCF2 repressor element (HpaII site 4) (see Figure 4A). Embryonic stem (ES) cells were transfected and selected for neomycin resistance, and positive clones were assayed by Southern blot hybridization for targeting events. In eight out of 480 clones we detected an insertion in the genomic Igf2 locus (Figure 4B). The resulting ES clones were injected into blastocysts, and chimeras were used to generate mouse lines with the mutated gene in the germline. These were then crossed with a cre-expressing mouse in order to remove the Neor gene cassette (Lallemand et al., 1998). PCR amplifications of DNA from these clones and subsequent sequencing confirmed that the GCF2 element was indeed disrupted by the presence of a single loxP sequence in one copy of the Igf2 gene (data not shown). Figure 4.Genomic targeting of a loxP element into DMR1 HpaII site 4. (A) The targeting construct. A 5 kb BamHI fragment containing DMR1 was subcloned from a genomic library and a loxP-Neor-loxP cassette (bottom line) was then inserted into HpaII site 4, which resides within a GCF2 consensus sequence (see Kageyama and Pastan, 1989; Reed et al., 1998). (B) Southern blot analysis of genomic DNA from wild-type (WT), targeted and cre-deleted ES cells restricted with BglII and probed with the fragments indicated in the map. Only data from the left hand probe are shown in the blot. The targeted allele appears under-represented in this figure because of the presence of DNA from accompanying feeder cells. The nature of the disruption was verified by amplifying and sequencing this region from the cre-deleted mice. Download figure Download PowerPoint In normal mice, the Igf2 gene is repressed on the maternal allele. It was thus of interest to determine how much of this repression may be due to the presence of the upstream GCF2 element located at HpaII site 4. In order to enable the measurement of transcription exclusively from the maternal allele, we utilized mice in which the paternal allele was deleted. To this end, female mice heterozygous for the repressor mutation (R+/−) were mated to males heterozygous for an Igf2 deletion (Igf2+/−) (DeChiara et al., 1990). Embryos or newborn progeny were then genotyped by PCR analysis and the Igf2−R+/Igf2+R− or Igf2−R+/Igf2+R+ animals then assayed for the level of Igf2 mRNA using quantitative RT–PCR. Initial expression studies on individual whole embryos (12.5–17.5 d.p.c.) showed that the R− mutation had only a small (2.8-fold) but statistically significant (P <0.001) effect (see legend to Figure 5). In order to average out the small variations between embryos, we collected pools of 8–10 embryos for each genotype, and then measured the degree of Igf2 transcription relative to an internal β-actin control. As shown in Figure 5A, these experiments indicated that ∼3-fold more mRNA is made from a gene carrying the repressor element mutation as compared with the wild-type maternal allele. These expression studies were also confirmed by employing an RNase protection assay on the same tissue samples (data not shown). These findings in whole embryos mainly reflect the expression pattern in mesoderm, but similar results were also obtained using RNA derived from placenta or yolk sac and from individual endoderm-derived tissues, such as liver (data not shown). This constitutive pattern, as well as the low magnitude of inhibition, suggests that the GCF2 element is a newly discovered repressor that is independent of the, as yet undefined, sequences responsible for the mesoderm- specific repression previously identified through targeted deletion of a large 5 kb region surrounding DMR1 (Constancia et al., 2000). Figure 5.Analysis of Igf2 maternal allele expression in targeted mice. (A) RT–PCR on total RNA extracted from 8–10 whole 12.5 d.p.c. embryos, which were first genotyped by PCR in order to identify and group the R+/Igf2− and R−/Igf2− individuals. PCR was carried out on 1, 3 or 9 μl of cDNA in the presence of [α-32P]dCTP, and the products were run on a 5% acrylamide gel. PCR for β-actin cDNA was carried out in the same tube and served as a control. Igf2 transcription in R− embryos was 2.9-fold higher than in R+ embryos as determined by phosphoimager analysis, after normalization for β-actin. Similar results were also obtained using pooled embryos from an additional inde pendent targeted mouse line. We also carried out expression analysis on individual embryos. In this case, the level of Igf2 mRNA was normalized against β-actin, and the average relative amount of Igf2 in the R+ embryos set at 1 (R+, 1.0 ± 0.2, n = 5; R−, 2.8 ± 0.6, n = 6, P <0.001). (B) RT–PCR was carried out on total RNA from 8–10 whole 12.5 d.p.c. embryos obtained from crosses between M.musculus mice carrying one targeted Igf2 allele (R−) and homozygous SD7 mice (R+). The PCR product was then cut with BsaAI, which recognizes a polymorphic site present only in the M.spretus allele, and was run on a 7% acrylamide gel. In the left panel, the R− allele is derived from the mother while, in the right panel, this allele is paternally derived. The R− maternal allele is 2.8-fold more active than the R+ allele when compared with the spretus paternal allele, as determined by phosphoimager analysis. Download figure Download PowerPoint Another way to distinguish between the maternal and paternal Igf2 gene products is to use mice containing one allele derived from M.musculus and the second from M.spretus. To this end, we carried out reciprocal crosses between M.m.domesticus R−/+ and homozygous SD7 mice, which have two copies of chromosome 7 whose distal portion is derived from M.spretus. Embryos were then assayed for Igf2 mRNA by RT–PCR and allele-specific expression revealed by using a restriction enzyme to detect a polymorphism in the spretus allele. As expected, the paternal allele is expressed at much higher levels than the maternal allele in all mice (Figure 5B). When the repressor mutation is present on the maternal allele, however, expression from this gene copy is elevated ∼3-fold. In contrast, when this mutation is present on the highly active paternal allele, it apparently has little effect on transcription (Figure 5B). It should be noted that the mutation itself did not influence the methylation pattern at other HpaII sites in DMR1 (data not shown). When taken together, these results clearly suggest that the upstream repressor element indeed plays a role in bringing about low expression levels from the maternal allele. Discussion Characterization of an Igf2 upstream repressor element The control of genomic imprinting is obviously a complicated process, which involves molecular decision making at multiple stages of development. These events leave in their wake clearcut footprints in the form of allele-specific DNA methylation (Razin and Cedar, 1994; Constancia et al., 1998). The methyl moieties not only serve as a means of differentially marking the two parental alleles, but also probably operate directly by influencing chromatin structure and gene expression. Here, we have studied the function of a single DMR located adjacent to the mouse Igf2 gene, and find that this region plays a novel role in the regulation of Igf2 imprinted expression. On the basis of a transient reporter gene transfection assay, we have identified an element within DMR1 that represses promoter activity 3- to 5-fold, and this sequence was deemed to be both necessary and sufficient for inhibiting transcription in this in vitro system. Furthermore, by employing gene targeting technology to introduce a site-specific mutation in vivo, it was clearly demonstrated that this same element actually also plays a role in the repression of the endogenous maternal Igf2 allele. Biochemical evidence indicates that this repr
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