Hob3p, the fission yeast ortholog of human BIN3, localizes Cdc42p to the division site and regulates cytokinesis
2007; Springer Nature; Volume: 26; Issue: 7 Linguagem: Inglês
10.1038/sj.emboj.7601641
ISSN1460-2075
AutoresPedro M. Coll, Sergio A. Rincón, Raul A Izquierdo, Pilar Pérez,
Tópico(s)Cellular transport and secretion
ResumoArticle15 March 2007free access Hob3p, the fission yeast ortholog of human BIN3, localizes Cdc42p to the division site and regulates cytokinesis Pedro M Coll Pedro M Coll Consejo Superior de Investigaciones Científicas (CSIC)/Departamento de Microbiología y Genética, Instituto de Microbiología Bioquímica, Universidad de Salamanca, Edificio Departamental, Salamanca, Spain Search for more papers by this author Sergio A Rincon Sergio A Rincon Consejo Superior de Investigaciones Científicas (CSIC)/Departamento de Microbiología y Genética, Instituto de Microbiología Bioquímica, Universidad de Salamanca, Edificio Departamental, Salamanca, Spain Search for more papers by this author Raul A Izquierdo Raul A Izquierdo Consejo Superior de Investigaciones Científicas (CSIC)/Departamento de Microbiología y Genética, Instituto de Microbiología Bioquímica, Universidad de Salamanca, Edificio Departamental, Salamanca, Spain Search for more papers by this author Pilar Perez Corresponding Author Pilar Perez Consejo Superior de Investigaciones Científicas (CSIC)/Departamento de Microbiología y Genética, Instituto de Microbiología Bioquímica, Universidad de Salamanca, Edificio Departamental, Salamanca, Spain Search for more papers by this author Pedro M Coll Pedro M Coll Consejo Superior de Investigaciones Científicas (CSIC)/Departamento de Microbiología y Genética, Instituto de Microbiología Bioquímica, Universidad de Salamanca, Edificio Departamental, Salamanca, Spain Search for more papers by this author Sergio A Rincon Sergio A Rincon Consejo Superior de Investigaciones Científicas (CSIC)/Departamento de Microbiología y Genética, Instituto de Microbiología Bioquímica, Universidad de Salamanca, Edificio Departamental, Salamanca, Spain Search for more papers by this author Raul A Izquierdo Raul A Izquierdo Consejo Superior de Investigaciones Científicas (CSIC)/Departamento de Microbiología y Genética, Instituto de Microbiología Bioquímica, Universidad de Salamanca, Edificio Departamental, Salamanca, Spain Search for more papers by this author Pilar Perez Corresponding Author Pilar Perez Consejo Superior de Investigaciones Científicas (CSIC)/Departamento de Microbiología y Genética, Instituto de Microbiología Bioquímica, Universidad de Salamanca, Edificio Departamental, Salamanca, Spain Search for more papers by this author Author Information Pedro M Coll1,‡, Sergio A Rincon1,‡, Raul A Izquierdo1 and Pilar Perez 1 1Consejo Superior de Investigaciones Científicas (CSIC)/Departamento de Microbiología y Genética, Instituto de Microbiología Bioquímica, Universidad de Salamanca, Edificio Departamental, Salamanca, Spain ‡These authors contributed equally to this work *Corresponding author. Consejo Superior de Investigaciones Científicas (CSIC)/Departamento de Microbiología y Genética, Instituto de Microbiología Bioquímica, Universidad de Salamanca. Edificio Departamental, Salamanca 37007, Spain. Tel.: +34 923 121644; Fax: +34 923 224876; E-mail: [email protected] The EMBO Journal (2007)26:1865-1877https://doi.org/10.1038/sj.emboj.7601641 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Cdc42 GTPase is required for polarization in eukaryotic cells, but its spatial regulation is poorly understood. In Schizosaccharomyces pombe, Cdc42p is activated by Scd1p and Gef1p, two guanine-nucleotide exchange factors. Two-hybrid screening identified Hob3p as a Gef1p binding partner. Hob3p is a BAR domain-containing protein ortholog of human Bin3. Hob3p also interacts directly with Cdc42p independently of Gef1p. Hob3p, Cdc42p and Gef1p form a complex, and Hob3p facilitates Gef1p–Cdc42p interaction and activation. Hob3p forms a ring in the division area, similar to that of Gef1p. This localization requires actin polymerization and Cdc15p but is independent of the septation initiation network. Hob3p is required for the concentration of Cdc42p to the division area. The actomyosin ring contraction is slower in hob3Δ than in wild-type cells, and this contributes to its cytokinesis defect. Moreover, this report extends previous evidence that human Bin3 suppresses the cytokinesis phenotype of hob3Δ cells, showing that Bin3 can partially recover the GTP-Cdc42p level and its localization. These results suggest that Hob3p is required to recruit and activate Cdc42p at the cell division site and that this function might be conserved in other eukaryotes. Introduction Schizosaccharomyces pombe is a highly polarized yeast growing by elongation of its ends and dividing by medial septation. Cytokinesis begins with the assembly of a contractile actomyosin medial ring attached to the cell membrane (Feierbach and Chang, 2001; Guertin et al, 2002). Most of the components of this ring are conserved among animal cells (Glotzer, 2005). The septation initiation network (SIN) triggers the contraction of the actin ring and the consecutive addition of new membranes and synthesis of the primary septum in coordination with the nuclear cycle (Balasubramanian et al, 2004; Krapp et al, 2004; Wolfe and Gould, 2005). BAR (Bin–Amphiphysin–Rvs) domain-containing proteins are a well-conserved family that includes Rvs proteins in yeast, and amphiphysins and BIN proteins in mammals. BAR family members integrate signal pathways that regulate membrane dynamics, F-actin cytoskeleton, and nuclear processes (Peter et al, 2004; Zimmerberg and McLaughlin, 2004; Ren et al, 2006). There are three main functions associated with the BAR domain: (1) sensing and/or induction of membrane curvature at endocytic sites (Peter et al, 2004; Zimmerberg and McLaughlin, 2004), (2) binding to small GTPases (Habermann, 2004), and (3) transcriptional repression properties (Elliott et al, 1999; Miaczynska et al, 2004). Saccharomyces cerevisiae RVS161 and RVS167 genes, coding for two BAR domain-containing proteins, were isolated in a screening for mutations causing reduced viability upon nutrient starvation. Rvs161p and Rvs167p regulate endocytosis, vesicle traffic, F-actin organization, and cell polarity (Ren et al, 2006). These two proteins form heterodimers through their respective BAR domains. Rvs167p contains other domains and interacts with many proteins that have well-characterized roles in the actin cytoskeleton organization, including Abp1p, Acf2p, Act1p, Las17p, Sla1p, Sla2p, and Srv2p (Ren et al, 2006). In addition, RVS167 shows synthetic lethal or synergistic negative growth interactions with MYO1, MYO2, and ACT1 as well as with SLT2 and KRE6 (Breton et al, 2001). Rvs161p is required for actin repolarization upon salt stress and is associated with lipid rafts (Balguerie et al, 2002). S. pombe also has two BAR domain proteins, Hob1p, structurally similar to Rvs167/amphiphysin (Routhier et al, 2003), and Hob3p, similar to human BIN3 and Rvs161p (Routhier et al, 2001). Surprisingly, neither Hob1p nor Hob3p is required for endocytosis, unlike Rvs167p and Rvs161p. hob1+ deletion causes totally different phenotypes than those produced by the deletion of the budding yeast RVS167. hob1Δ cells are slightly elongated, fail to arrest upon nutrient starvation, and are hypersensitive to genotoxic stress. These defects were complemented by human BIN1 (Routhier et al, 2003). S. pombe cells lacking Hob3p are multiseptated and the F-actin patches are distributed randomly around the cell. Interestingly, the actin localization defects of hob3Δ mutants were rescued by human BIN3 and only partially rescued by RVS161 (Routhier et al, 2001). These findings suggest that hob3+ and BIN3 are orthologs, and have diverged from RVS161 during evolution. Cdc42 GTPase is a key molecule in establishing cell polarity in eukaryotic cells. Like other GTPases, Cdc42p acts as a molecular switch, cycling between an inactive (GDP-bound) and an active (GTP-bound) state. The local activation of Cdc42p at the growth site is required for actin polymerization and growth establishment (Etienne-Manneville, 2004). Fission yeast Cdc42p is essential for cell proliferation and also for maintenance of the rod-like cell morphology (Miller and Johnson, 1994). Cdc42p is mainly localized to the medial region and growing tips of the cell. It also localizes to the periphery and internal membranes (Merla and Johnson, 2000). Cdc42p is activated by two GTPase nucleotide exchange factors (GEFs): Scd1p, required to maintain the apical growth (Chang et al, 1994); and Gef1p, which plays a role in cytokinesis and the switch to bipolar growth (Coll et al, 2003; Hirota et al, 2003). The scaffold protein Scd2p allows the interaction between Cdc42p, the exchange factor Scd1p, and the p21-activated kinase Shk1p that regulates apical growth (Chang et al, 1999). As mentioned, Cdc42p localizes to the medial region, but a possible role of Cdc42p in S. pombe cytokinesis has not been described. Additionally, it is not known if other scaffold proteins, different from Scd2p, would cause the local activation of Cdc42p in the division area. Here we describe the interaction of Gef1p and Cdc42p with the BAR domain-containing protein Hob3p. This protein concentrates Cdc42p to the medial region and it is also necessary for the Gef1p-mediated Cdc42p activation. We also show that overexpression of Bin3 can substitute for Hob3p, suggesting that they have a function conserved among eukaryotes. Results Hob3p interacts with Gef1p It has been shown that Gef1p is a Cdc42-specific exchange factor that participates in the regulation of cytokinesis (Coll et al, 2003; Hirota et al, 2003). Gef1p contains a Dbl homology (DH) domain responsible for their exchange activity, but lacks a pleckstrin homology (PH) domain. Gef1p does not interact with any of the known proteins from the Cdc42p signaling pathway that interact with Scd1p, such as Ras1p, Scd2p, Shk1p, or Shk2p (Hirota et al, 2003; our unpublished results). In order to identify the possible proteins of the Gef1p-Cdc42p signaling pathway, we performed a two-hybrid screen using Gef1p as bait, and 12 positive clones were obtained. Six of them contained the sequence coding for Hob3p, a BAR domain-containing protein, ortholog of human Bin3 and S. cerevisiae Rvs161p (Routhier et al, 2001). To map the Gef1p interaction with Hob3p, different Gef1p coding fragments were used as bait with pAS2-hob3+. gef1 constructs lacking the sequence coding the C-terminal 243 amino-acid residues failed to associate with Hob3p, suggesting that this region is required for the interaction with Hob3p (Figure 1A). Figure 1.Gef1p interacts with Hob3p. (A) Two-hybrid analysis of the interactions between different fragments of Gef1p (pACT2) and Hob3p (pAS2). (B) Cells carrying endogenous hob3-myc were transformed with pREP1-GST-gef1+ and grown in the absence of thiamine for 12 h. Cell extracts were pulled down with GS beads and the precipitates were probed with anti-Myc antibody (top). Extracts were also analyzed by Western blot using anti-Myc (9E10) or anti-GST antibodies (bottom panels). (C) Extracts from cells carrying endogenous gef1-HA and hob3-GST were pulled down with GS beads and the precipitates were probed with anti-HA antibody. Extracts from gef1-HA cells transformed with the plasmid pREP-GST were used as negative control. Download figure Download PowerPoint To verify the interaction between Gef1p and Hob3p, we performed co-immunoprecipitation experiments using cells transformed with the plasmid pREP-GST-Gef1p and we showed that GST-Gef1p co-precipitated with Myc-Hob3p expressed from the endogenous hob3+ promoter (Figure 1B). Additionally, we demonstrated that Hob3-GST and Gef1-HA, both expressed from the endogenous promoters, co-immunoprecipitated (Figure 1C). Together, these results indicate that Gef1p and Hob3p physically associate in S. pombe cells. To see if Hob3p was required for Gef1p localization, we analyzed Gef1-GFP in hob3Δ cells, and observed that Gef1-GFP localized to the septation area, as in wild-type cells (data not shown). Therefore, Hob3p was not necessary for Gef1p localization. We also looked for any genetic interaction between gef1+ and hob3+. The absence of Gef1p causes an increase in septating cells (Coll et al, 2003), and aggravates the phenotype of other cytokinesis mutants (our unpublished results). Elimination of Hob3p causes an increase in septating cells and the appearance of some multiseptated cells. hob3Δ gef1Δ double mutant was viable at all temperatures and the percentage of cells with one or more septa was similar to hob3Δ (40% septating cells; 9% multiseptated cells; 7% elongated cells at 32°C, n=550, for hob3Δ cells and 42% septating cells; 9% multiseptated cells; 8% elongated cells at 32°C, n=720 for the hob3Δ gef1Δ cells). These results suggest that Hob3p and Gef1p are in the same signaling pathway. Hob3p interacts with Cdc42p Hob3p belongs to the BAR domain-containing protein family, and some members of this family can bind to small GTPases (Habermann, 2004). Hence, Hob3p could be an effector of Cdc42p, interacting with this GTPase when activated by Gef1p. To test this possibility, we analyzed whether Hob3p also binds to Cdc42p. Co-precipitation experiments were performed using extracts from cells expressing GST-hob3 and HA-cdc42 from their respective endogenous promoters. Interestingly, Hob3p co-precipitated with Cdc42p in both wild-type and gef1Δ cells (Figure 2A); therefore, Gef1p is not required for this interaction. Additionally, co-precipitation experiments in cells transformed with either the constitutively active cdc42G12V allele or the dominant-negative cdc42T17N allele showed that Hob3p interacted with Cdc42p independent of its activation state (Figure 2B). Taken together, these results suggest that Hob3p is not a Cdc42p effector but directly associates with this GTPase. Figure 2.Hob3p interacts with Cdc42p and Gef1p. (A) Extracts from wild-type and gef1Δ cells carrying hob3-GST and HA-cdc42 expressed under their endogenous promoters were pulled down with GS beads and the precipitates were probed with anti-HA antibody (top). Extracts were assayed for expression of Hob3 and Cdc42 by Western blot analysis using anti-GST or anti-HA (12CA5) antibodies (bottom). (B) Cells expressing endogenous hob3-GST were transformed with pREP42-HA-Cdc42, pREP42-HA-Cdc42G12V, or pREP42-HA-Cdc42T17N and grown in the absence of thiamine for 12 h. Cell extracts were pulled down with GS beads and the precipitates were probed with anti-HA antibody. Hob3-GST and plasmid expression was analyzed by Western blot. (C) Gef1p, Cdc42p and Hob3p form an in vivo complex. hob3-GFP- and HA-cdc42-expressing cells were transformed with either pREP-GST-gef1+ or pREP-GST and grown in the absence of thiamine for 12 h. Cell extracts were immunoprecipitated with GS beads and blotted with anti-GFP antibody for the presence of Hob3-GFP (left), stripped and blotted with anti-HA antibody to detect HA-cdc42 (right). (D) hob3-GFP and hob3Δ cells expressing HA-cdc42 were transformed with pREP-GST-gef1+ and grown in the absence of thiamine for 12 h. Cell extracts were subjected to size-exclusion chromatography (Superdex 200) and fractions were analyzed by SDS–PAGE and blotted with the corresponding antibodies. Fractions 21–36 are shown. A portion of GST-Gef1p, Hob3-GFP, and HA-cdc42p co-migrated in 250–300 kDa molecular mass fractions (box). The standard molecular weight proteins and the UV absortion chromatograms of the extracts (wt cells darker and hob3Δ cells lighter) are shown in the top and bottom panels, respectively. Download figure Download PowerPoint As Gef1p, Hob3p, and Cdc42p interact with each other, we considered the possibility that Hob3 might facilitate Gef1p–Cdc42p interaction. Therefore, we analyzed if the three proteins coexisted in a complex. Cells expressing hob3-GFP and HA-cdc42 from the respective endogenous promoters were transformed with the pREP-GST-gef1 plasmid. Moderate expression of the plasmid was induced and co-precipitation of the three proteins by pull-down with gluthation-Sepharose beads was successful (Figure 2C). These experiments suggest that a complex of Gef1p–Hob3p–Cdc42p may be present in vivo. To further investigate if the three proteins coexist in a complex, extracts from hob3-GFP HA-cdc42 and hob3Δ HA-cdc42 cells transformed with the pREP-GST-gef1 plasmid were fractionated by gel filtration (Figure 2D). HA-cdc42p has a predicted mass of 23 kDa; however, the elution position of HA-cdc42p from the column was that expected for a protein of much bigger size. Similarly, Hob3-GFP (predicted mass of 57 kDa) and GST-gef1p (predicted mass of 110 kDa) appear in fractions expected for proteins of bigger size. A small portion of the three proteins co-fractionated, suggesting that they might form part of a complex of 250–300 kDa. Most Cdc42p appears in higher molecular weight fractions, probably because in a logarithmic culture only 15–20% of the cells are septating and Cdc42p also forms complex with other signaling proteins, such as Scd1p and Scd2p, at the growing tips. In the absence of Hob3p, GST-Gef1p shifted toward lower molecular weight, and HA-cdc42p appears more spread throughout the column (fractions 21–28). The absence of Hob3p affects Cdc42p and Gef1p mobility, further supporting that they form part of the same complex. Hob3p is necessary for the Gef1p-dependent activation of Cdc42p We next analyzed whether Hob3p could be necessary for the Gef1p interaction with Cdc42p. Gluthation-Sepharose (GS) beads pull-down of cell extracts from wild-type and hob3Δ cells endogenously expressing HA-cdc42 and GST-gef1 were performed. HA-Cdc42p was present in both wild-type and hob3Δ cell pull-downs, but the level in hob3Δ cells was half that of the wild-type cells (Figure 3A). These results suggest that Hob3p facilitates the interaction of Gef1p with Cdc42p, although is not absolutely required for it. To further investigate whether Hob3p was necessary for Gef1p activation of Cdc42p, we analyzed the amount of active GTP-bound HA-Cdc42p in wild-type, hob3Δ, gef1Δ, and gef1Δhob3Δ double mutant cells. As expected, the levels of GTP-Cdc42p in gef1Δ cells were 30% that of wild-type cells. The lack of Hob3p also decreased the levels of GTP-Cdc42p to 45% that of wild-type cells (Figure 3B). Interestingly, hob3Δ gef1Δ cells had GTP-Cdc42p levels similar to gef1Δ cells (Figure 3B), suggesting that Hob3p and Gef1p are in the same Cdc42 activating pathway. Additionally, whereas the overexpression of Gef1p considerably increased GTP-Cdc42p, Hob3p overexpression did not increase the active Cdc42p levels (Figure 3C). These results suggest that Hob3p is not a Cdc42-GEF, but is necessary for Cdc42p activation. To test genetically whether Hob3p was necessary for the function of Gef1p, we made the hob3Δ scd1Δ double mutant strain. The double mutant gef1Δscd1Δ lacking both Cdc42p activators is lethal (Coll et al, 2003; Hirota et al, 2003). Therefore, if Hob3p were necessary for Gef1p function, hob3Δ scd1Δ would be lethal as well. scd1+ gene was replaced by KanMX6 in a hob3+/hob3∷ura4+ diploid strain and 44 tetrad spore analysis showed that the ura+ spores (hob3Δ) that germinated and proliferated were never resistant to kanamycin (scd1Δ). The spores Kanr ura+ germinated and underwent two or three cell divisions before arresting as small rounded cells (Figure 3D), similar to the spores of the double mutant gef1Δ scd1Δ (Coll et al, 2003). Taken together, these results indicate that Hob3p facilitates Gef1p–Cdc42p interaction and is necessary for Cdc42p activation mediated by Gef1p; consequently, Hob3p function is essential in the absence of Scd1p, the other Cdc42p activator. Figure 3.Hob3p is required for Cdc42p activation mediated by Gef1p. (A) Extracts from wild-type and hob3Δ cells carrying GST-gef1+ and HA-Cdc42 expressed under their own promoters were pulled down with GS beads and the precipitates were probed with anti-HA antibody. Extracts were assayed for expression of HA-Cdc42 and GST-gef1 by Western blot. Data were quantified and presented as percentage relative to the control extracts run in the same experiment. (B) HA-cdc42-expressing wild-type, gef1Δ, hob3Δ, and hob3Δ gef1Δ cells were analyzed for total HACdc42p by Western blot using 12CA5 anti-HA monoclonal antibody and for GTP-bound HA-Cdc42p by pulling down the cell extracts with GST-CRIB beads and blotting against 12CA5. Data were quantified and presented as percentage relative to the total HA-Cdc42 controls. (C) Wild-type cells transformed with pREP4X, pREP3X-hob3, or pREP4X-gef1+ were analyzed for total HA-Cdc42p and for GTP-bound HA-Cdc42p as in (B). (D) Tetrad analysis of the diploid strain hob3+/hob3∷ura4+ scd1+/scd1∷KanMX6 and a micrograph of hob3Δscd1Δ microcolonies formed after spore germination. Download figure Download PowerPoint Hob3p forms a ring at the medial region and localizes to patches at the growing poles To gain further insight into the Hob3p relationship with Cdc42p and Gef1p, we first examined the subcellular localization of Hob3-GFP. The genomic locus of hob3+ was tagged with the GFP gene fused in-frame to the 3′ end of hob3+ open reading frame (ORF). Hob3-GFP was observed in the medial region (Figure 4A, left). Confocal microscopy demonstrated that Hob3p formed a ring in that region (Figure 4A, right). To analyze Hob3p localization during septation, we constructed the double mutant cdc25-22 hob3-GFP and synchronized cells in G2 phase by arrest at 36.5°C for 4 h. Cells were returned to permissive temperature (25°C) and samples were taken at different time points to observe Hob3-GFP and calcofluor staining. Hob3-GFP appeared in the medial region before the septum was formed (60 min), remained during the ring contraction and septum formation, and disappeared by cell separation (Figure 4B). Figure 4.Hob3p localizes to the division area and patches at the growing poles. (A) Fluorescence microscopy of hob3-GFP cells grown to mid-log phase (left panel) and examined by confocal microscopy (right panel). (B) cdc25-22 hob3-GFP cells were grown at 25°C to log phase, then shifted to 36.5°C for 4 h to block cells in G2, and shifted back to 25°C. Cells were taken at different time points, stained with calcofluor, and examined for GFP and calcofluor fluorescence (CF). (C) cdc10-129 hob3-GFP cells were grown at 25°C to log phase, then shifted to 36.5°C and observed after 4 h. (D) gef1Δ hob3-GFP cells grown to mid-log phase. The bar corresponds to 5 μm. Download figure Download PowerPoint Hob3p was also localized as patches at one or both poles in non-septating cells. cdc10-129 mutant cells expressing hob3-GFP were used to see if Hob3-GFP patches accumulate in the single growing pole. Cells were grown at 25°C and shifted to 36.5°C. At this temperature, cdc10-129 cells arrest in G1 and grow only through the old tip. In those cells, Hob3-GFP patches accumulate in the growing pole (Figure 4C). We also examined whether Gef1p was required for proper localization of Hob3p, but we did not observe any difference in Hob3-GFP localization in gef1Δ cells compared to wild-type cells (Figure 4D). Therefore, Hob3p and Gef1p do not depend on each other to localize correctly. Hob3p localization is actin dependent It has been described that the absence of Hob3p causes mislocalization of actin patches (Routhier et al, 2001). To see whether Hob3p patches contained actin, we examined rhodamine–phalloidin actin staining and Hob3-GFP simultaneously. Hob3-GFP patches overlapped some of those recognized by rhodamine–phalloidin, suggesting that Hob3p localizes to some actin patches at the growth poles (Figure 5A). Hob3-GFP ring also seemed to colocalize with the actin ring (Figure 5B). However, the overlap is not total. It has been described that Gef1p localizes to a contractile ring that resides immediately outside of the contractile actomyosin ring (Coll et al, 2003; Hirota et al, 2003). It is possible that Hob3p localizes to the same Gef1p ring. We constructed a strain carrying gef1-GFP and hob3-RFP and, although both proteins are difficult to visualize, the results showed that there was extensive overlapping between Hob3-RFP and Gef1-GFP in a contractile ring during cytokinesis (Figure 5C). Gef1p ring depends on the integrity of the contractile ring and it is dispersed by Latrunculin A treatment (Hirota et al, 2003). We examined whether Hob3p localization was also dependent on actin polymerization by promoting the disassembly of actin using 20 μM Latrunculin A. After 5 min with Latrunculin A, most actin was depolymerized, and Hob3-GFP, either from the middle region or from the growing pole patches, was dispersed to a diffuse cytoplasmic fluorescence (Figure 5D). Therefore, Hob3p localization is actin dependent. Figure 5.Hob3p localization depends on actin polimerization. (A) Colocalization of Hob3p and F-actin. hob3-GFP cells, grown to mid-log phase were stained with rhodamine–phalloidin and examined by fluorescence microscopy. (B) Three cells are shown in more detail to see the contractile rings. (C) Colocalization of Hob3-RFP and Gef1-GFP. Three cells are shown in detail to see the contractile rings. (D) Localization of Hob3-GFP depends on F-actin integrity. Fluorescence microscopy of cells grown to mid-log phase and incubated for 5 min in the presence of 1% dimethyl sulfoxide (DMSO) (top) or 20 μM Latrunculin A (LatA) bottom). Cells of the same culture were fixed and stained with rhodamine–phalloidin to observe actin localization. The bar corresponds to 5 μm. Download figure Download PowerPoint Hob3p localization to the division area is dependent on Cdc15p but not on the SIN pathway To further investigate how Hob3p localization is regulated, we analyzed Hob3-GFP in cdc15-140 mutant strain. Cdc15p is an FCH domain-containing protein necessary for the actomyosin ring formation (Fankhauser et al, 1995). cdc15-140 cells are impaired in the assembly of the actomyosin ring when grown at 36.5°C (Carnahan and Gould, 2003). Hob3-GFP was not recruited to the medial region in cdc15-140 cells grown at the restrictive temperature. By contrast, Hob3-GFP patches at the cell poles were still observed at 36.5°C (Figure 6A). When cdc15-140 cells were switched from 36.5°C to the permissive temperature, Hob3-GFP was observed in the medial region of all septating cells (Figure 6A). These results suggest that the actomyosin ring could serve as a spatial landmark to target Hob3p to the division site. Figure 6.Hob3p localization to the division area depends on the actomyosin ring and is independent of the SIN pathway. (A) Fluorescence microscopy of cdc15-140 hob3-GFP. Cells were grown at 25°C to log phase, then shifted to 36.5°C for 4 h to block cells at cytokinesis (upper panel) and shifted back to 25°C (lower panel). (B) Fluorescence microscopy of cdc11-119 hob3-GFP cells grown at 25°C to log phase, then shifted to 36.5°C for 6 h. Cells were stained with calcofluor before observation. Download figure Download PowerPoint We also analyzed Hob3-GFP in cdc11-119 mutant cells to see if Hob3p localization required the SIN, essential for the formation of the division septum after assembly of the actomyosin ring (Wolfe and Gould, 2005). Hob3-GFP localized properly in cdc11-119 cells grown at the restrictive temperature (Figure 6B). These observations indicate that Hob3p localization is not dependent on the SIN pathway. Hob3p is required to localize Cdc42p to the division area It has been speculated that Gef1p and Scd1p may play a key role not only in activating Cdc42p but also recruiting it to the septation site (Hirota et al, 2003). As Hob3p is necessary for Gef1p function, we considered the possibility that Hob3p was required to localize Cdc42p to the division area. We constructed a strain carrying endogenously GFP-tagged Cdc42p. Cells with GFP-Cdc42p were viable although slightly misshapen, indicating that GFP-Cdc42p was not completely functional. Endogenous Cdc42p localization was similar to that described using Cdc42p overexpressed from a plasmid (Merla and Johnson, 2000). It localizes to the periphery of the cell and to internal membranes, with a higher concentration at the growth poles and the division area, where it first forms a ring that closes to form a disc (Figure 7 and Supplementary movie S1). Similar localization was observed in cells lacking either Scd1p or Gef1p, although the GFP-Cdc42 signal in the latter was weaker and non-uniform (Figure 7). By contrast, in cells lacking Hob3p, we could not observe GFP-Cdc42p concentrated at the division area. Cdc42 was still mildly visible at the growth poles (Figure 7 and Supplementary movie S2). S. pombe contains another protein from the BAR family, Hob1p, ortholog of human Bin1 (Routhier et al, 2003). We examined GFP-Cdc42p localization in hob1Δ cells to see if the other protein containing a BAR domain could also regulate Cdc42p. However, GFP-Cdc42p was perfectly localized to the division site in hob1Δ cells (Figure 7 and Supplementary movie S3), suggesting that the effect on Cdc42p localization is specific to Hob3p. Western blot analysis of GFP-Cdc42p in total cell extracts showed that the lack of GFP-Cdc42p staining in the septum area of hob3Δ cells was not due to a decrease in the total amount of Cdc42p (data not shown). Taken together, these results indicate that Hob3p localizes to the actin ring and is specifically required to localize Cdc42p to the division site. Figure 7.Hob3p is required for Cdc42p localization to the division area. GFP fluorescence micrographs of wild-type, hob3Δ, scd1Δ, gef1Δ, and hob1Δ cells with the endogenous cdc42 gene tagged with GFP. The bar correspond
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