Qualitative and quantitative reversed-phase high-performance liquid chromatography of flavonoids in Passiflora incarnata L.
1994; Elsevier BV; Volume: 69; Issue: 3 Linguagem: Inglês
10.1016/0031-6865(94)90017-5
ISSN1879-0194
AutoresA. Rehwald, Beat H. Meier, Otto Sticher,
Tópico(s)Medicinal Plants and Neuroprotection
ResumoA qualitative and a quantitative reversed-phase high-performance liquid chromatographic (HPLC) method for the analysis of flavonoids in Passiflora incarnata have been developed. They allow the isocratic separation of the major C-glycosylflavones occurring in this plant. By the fingerprint analysis of fourteen different samples a great variability of the qualitative composition as well as considerable differences concerning the relative concentrations of the flavonoidscould be demonstrated. The quantitative determination is based on acid hydrolysis of a methanolic extract with subsequent HPLC analysis. The major peaks after acid hydrolysis are isovitexin, isoorientin, schaftoside and isoschaftoside, which are calculated as isovitexin. The results obtained by using this method were twice as high as by applying the usual spectrophotometric method. Swertisin was isolated from a methanolic extract of P. incarnata and identified on the basis of ultraviolet, mass and 1H and 13C nuclear magnetic resonance spectral data. It is the first time that swertisin has been found in this plant.
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