Structure of in‐serum transfecting DNA–cationic lipid complexes
1997; Wiley; Volume: 409; Issue: 2 Linguagem: Inglês
10.1016/s0014-5793(97)00505-x
ISSN1873-3468
AutoresTsiala Boukhnikachvili, Olivier Aguerre-Chariol, Marc Airiau, Sylviane Lesieur, Michel Ollivon, J Vacus,
Tópico(s)DNA and Nucleic Acid Chemistry
ResumoNoticeable modifications of in‐serum transfection efficiency of dioctadecylamidoglycyl‐spermine (DOGS)–DNA complexes are observed, depending on DNA condensation conditions. The structures of the complexes are studied, keeping in mind the variability of lipid polymorphism, by cryo‐transmission electron microscopy and X‐ray diffraction. By increasing both pH and ionic strength, well‐organised lamellar structures with a period of 65 Å replace supramicellar aggregates. A relationship between the structures and their in‐vitro transfection activity is established. Efficiency in the presence of serum is maintained when a lamellar arrangement is involved.
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