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Seroepidemiology of human group C rotavirus in the UK

1997; Wiley; Volume: 52; Issue: 1 Linguagem: Inglês

10.1002/(sici)1096-9071(199705)52

1096-9071

Vivienne L. A. James, Paul R. Lambden, E O Caul, Susan J. Cooke, Ian N. Clarke,

Biomedical and Engineering Education

The gene coding for the major inner capsid protein VP6 of human group C rotavirus was cloned into baculovirus using the pBlueBac2TM vector and expressed in insect cells. When cultured in High FiveTM cells, VP6 was expressed at a high level and exported to the cell culture medium. Purified VP6 was used to immunise rabbits. Hyperimmune rabbit serum, which reacted with native human group C rotavirus in infected cells, was used to develop and optimise an EIA for the detection of antibodies to group C rotavirus using the recombinant VP6 as a source of antigen. In a local epidemiological survey of 1000 sera grouped by age, an average of 43% of samples were found to have antibodies to human group C rotavirus with the highest proportion (66%) in the 71–75 year age group. In comparison, 97% of adults and 85% of children had antibodies to recombinant VP6 from the bovine RF strain of group A rotavirus. These results suggest that infection with human group C rotavirus is a common occurrence despite the apparent rarity of reports of human group C rotavirus in clinical samples from patients with gastroenteritis. J. Med. Virol. 52:86–91, 1997. © 1997 Wiley-Liss, Inc.