Response of ciliated epithelium to ozone and sulfuric acid
1980; Elsevier BV; Volume: 22; Issue: 2 Linguagem: Inglês
10.1016/0013-9351(80)90149-8
ISSN1096-0953
AutoresElaine C. Grose, Donald E. Gardner, Frederick J. Miller,
Tópico(s)Statistical Methods in Clinical Trials
ResumoThe effects of ozone (O3), sulfuric acid (H2SO4), and their interaction on ciliary activity were investigated. Following in vivo exposure to various concentrations of O3 and H2SO4, ciliary activity of isolated tracheal ring cultures was microscopically determined under stroboscopic illumination. Assay of tracheal rings immediately after a 2-hr exposure to 880 μg H2SO4/m3 showed a significant decrease (P < 0.0001) in ciliary beating frequency from controls. Following 72 hr in vitro maintenance, there was still a significant depression (P < 0.01) in ciliary activity of treatment cultures. In vivo recovery studies indicated that ciliary activity had returned to the normal range 72 hr after exposure. Exposure to 196 μg O3/m3 for 3 hr resulted in no significant difference from controls in ciliary activity. Experiments designed to investigate the effects of a sequential exposure to O3 followed by H2SO4 indicated a significant decrease (P < 0.05) in the ciliary beating frequency of exposed animals which was less than that observed with H2SO4 alone. As indicated by the results of these studies, combined action experiments are extremely relevant in assessing the toxicity of environmental pollutants.
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