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Improved Laboratory Safety by Decontamination of Unstained Sputum Smears for Acid-Fast Microscopy

2005; American Society for Microbiology; Volume: 43; Issue: 8 Linguagem: Inglês

10.1128/jcm.43.8.4245-4248.2005

1098-660X

Luiz Roberto Bigão Giacomelli, César Helbel, R. Ogassawara, Ângela Maria Werneck Barreto, Fátima Moreira Martins, Celso Luíz Cardoso, Clarice Queico Fujimura Leite,

Innovative Microfluidic and Catalytic Techniques Innovation

Tubercle bacilli may survive in unstained heat-fixed sputum smears and may be an infection risk to laboratory staff. We compared the effectiveness of 1% and 5% sodium hypochlorite, 5% phenol, 2% glutaraldehyde, and 3.7% formalin in killing Mycobacterium tuberculosis present in smears prepared from 51 sputum samples. The smears were decontaminated by the tube and slide techniques. Phenol at 5%, glutaraldehyde at 2%, and buffered formalin at 3.7% for 1 min (tube technique) or for 10 min (slide technique) were effective in decontaminating sputum smears and preserved cell morphology and quantitative acid-fast microscopy results.