Localization of proteins forming the outer surface of isolated metaphase chromosomes
1992; Wiley; Volume: 297; Issue: 1-2 Linguagem: Inglês
10.1016/0014-5793(92)80323-9
ISSN1873-3468
AutoresN. D. Belyaev, Vladimir G. Budker, Veronika Dubrovskaya, Andrej A. Kim, Елена Киселева, Victor N. Sidorov,
Tópico(s)Muon and positron interactions and applications
ResumoThe outer surface of isolated metaphase chromosomes has been investigated by a method of thermally activated tritium labelling. We show that both chromosomal proteins and DNA are tritium-labelled. Fractionation of the chromosomal proteins reveals that scaffold proteins are the most labelled in condensed and EDTA-decondensed chromosomes. Exposition of some scaffold proteins on the outer surface of metaphase chromosomes is suggested.
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