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Effects of hyperbaric oxygen therapy on the rat intestinal mucosa apoptosis caused by ischemia-reperfusion injury

2007; Wiley; Volume: 27; Issue: 4 Linguagem: Inglês

10.1002/micr.20349

1098-2752

Paulo Roberto Bertoletto, Djalma José Fagundes, Manuel de Jesus Simões, Celina T. F. Oshima, Edna Frasson de Souza Montero, Ricardo Santos Simões, Anna Tereza Negrini Fagundes,

Cardiac Ischemia and Reperfusion

Abstract To examine the apoptosis expression in the intestinal mucosa in accordance of different periods of hyperbaric oxygen (HBO) treatment, rats were submitted to 60 min of mesenteric artery and vein ischemia and 60 min of reperfusion occlusion. A group (G‐IR) was the control and HBO was applied in the ischemia (GHBO‐I), reperfusion (GHBO‐R), and ischemia and reperfusion time (GHBO‐IR). After 60 min of reperfusion, samples of small bowel were prepared for immunohistochemical expression of caspase‐3. The expression of caspase‐3 was significantly inferior when HBO was administered in the ischemia (0.16 ± 0.01) in comparison with the control (0.70 ± 0.08), but HBO in the further reperfusion (0.84 ± 0.03) or both ischemia and reperfusion time (0.42 ± 0.05) was significantly worse. There was a connection between HBO, small bowel I/R injury, and mucosa apoptosis. The favorable effect was obtained when HBO was administered early in the ischemia time. © 2007 Wiley‐Liss, Inc. Microsurgery 2007.