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A noncytotoxic DsRed variant for whole-cell labeling

2008; Nature Portfolio; Volume: 5; Issue: 11 Linguagem: Inglês

10.1038/nmeth.1264

1548-7105

Rita Strack, Daniel E. Strongin, Dibyendu Bhattacharyya, Tao Wen, Allison Berman, Hal E. Broxmeyer, Robert J. Keenan, Benjamin S. Glick,

Virus-based gene therapy research

Many different red fluorescent proteins display cytotoxicity substantially higher than EGFP when used for whole-cell labeling of bacterial and mammalian cells with standard high-level expression systems. An improved tetrameric red fluorescent protein called DsRed-Express2 allows high-level labeling with minimal cytotoxicity comparable to that of EGFP. A common application of fluorescent proteins is to label whole cells, but many RFPs are cytotoxic when used with standard high-level expression systems. We engineered a rapidly maturing tetrameric fluorescent protein called DsRed-Express2 that has minimal cytotoxicity. DsRed-Express2 exhibits strong and stable expression in bacterial and mammalian cells, and it outperforms other available RFPs with regard to photostability and phototoxicity.