Antibody to threonyl–transfer rna synthetase in myositis sera
1988; Wiley; Volume: 31; Issue: 4 Linguagem: Inglês
10.1002/art.1780310408
ISSN1529-0131
AutoresIra N. Targoff, Frank C. Arnett, Morris Reichlin,
Tópico(s)Neurogenetic and Muscular Disorders Research
ResumoAbstract The prevalence and clinical correlations of antithreonyl–transfer RNA synthetase (anti–PL‐7), as well as the relationship of anti–PL‐7 to anti–histidyl–transfer RNA synthetase (anti–Jo‐1) were studied in 109 sera from patients with myositis. Inhibition of threonine aminoacylation was used to screen for anti–PL‐7. Sera from 3 patients, 2 with polymyositis and 1 with polymyositis–overlap syndrome, and a fourth serum from a patient with dermatomyositis, which was previously found to contain anti–PL‐7, inhibited >90% of activity (3.7% of 109 sera). All 4 sera reacted strongly in an enzyme‐linked immunosorbent assay with enzyme that was either affinity purified with anti–PL‐7 or was biochemically purified. There was no indication of cross‐reactivity by aminoacylation inhibition or, for most sera, by enzyme‐linked immunosorbent assay. Anti–PL‐7 is an uncommon myositis‐associated antibody that is independent of anti–Jo‐1, but is directed at a functionally related enzyme.
Referência(s)