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Simultaneous display of bacterial and fungal lipases on the cell surface of bacillus subtilis

2002; Elsevier BV; Volume: 93; Issue: 1 Linguagem: Inglês

10.1016/s1389-1723(02)80047-0

1389-1723

Gota Kobayashi, Kaori Fujii, Masakuni Serizawa, Hiroki Yamamoto, Junichi Sekiguchi,

Microbial Metabolic Engineering and Bioproduction

A small cell wall-binding domain (CWBc) of the Bacillus subtilis peptidoglycan hydrolase CwlC fused to B. subtilis lipase B was able to be localized on the cell wall of B. subtilis. With the aim of developing an efficient lipid-hydrolyzing bacterium with two different lipases on the cell surface, plasmids possessing genes for LipB-CWBc and CWBb-CutL (the cell wall-binding domain of CwlB fused to the Aspergillus oryzae lipolytic enzyme CutL) were constructed. B. subtilis harboring these plasmids accumulated considerable amounts of both lipases on the cell surface.