First Report of Pantoea agglomerans Causing Rice Leaf Blight in Venezuela
2014; American Phytopathological Society; Volume: 99; Issue: 4 Linguagem: Inglês
10.1094/pdis-07-14-0736-pdn
ISSN1943-7692
AutoresArias González, Marcos A. R. Franco, N. Contreras, Iván Galindo‐Castro, Yorman Jayaro, Eduardo Graterol,
Tópico(s)Banana Cultivation and Research
ResumoHomePlant DiseaseVol. 99, No. 4First Report of Pantoea agglomerans Causing Rice Leaf Blight in Venezuela PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Pantoea agglomerans Causing Rice Leaf Blight in VenezuelaA. D. González, M. A. Franco, N. Contreras, I. Galindo-Castro, Y. Jayaro, and E. GraterolA. D. González, M. A. Franco, N. Contreras, I. Galindo-Castro, Y. Jayaro, and E. GraterolAffiliationsAuthors and Affiliations A. D. González , Fundación para la Investigación Agrícola DANAC, San Javier, Estado Yaracuy, Venezuela M. A. Franco , Fundación Instituto de Estudios Avanzados (IDEA), Caracas, Venezuela N. Contreras , Decanato de Agronomía Universidad Centroccidental Lisandro Alvarado, Cabudare, Estado Lara, Venezuela I. Galindo-Castro , Fundación Instituto de Estudios Avanzados, (IDEA), Caracas, Venezuela Y. Jayaro , Fundación para la Investigación Agrícola DANAC, San Javier, Estado Yaracuy, Venezuela E. Graterol, Fondo Latinoamericano para Arroz de Riego (FLAR), Palmira, Colombia. Published Online:1 Apr 2015https://doi.org/10.1094/PDIS-07-14-0736-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat During the dry production seasons between 2002 and 2007, we collected leaf samples from Oryza sativa with clear symptoms of severe leaf blight in experimental fields and commercial crops in Guárico and Portuguesa States in Venezuela. On affected plants, disease severity ranged from 20 to 100%. Symptoms first appeared as yellowish or brownish lesions that later turned pale yellow and dry. Lesions eventually became necrotic. We consistently isolated a rod-shaped bacterium from diseased leaf tissues, which produced yellow colonies on nutrient agar. Colonies appearing after 24 h of incubation were transferred and replicated on nutrient agar and yeast extract, dextrose, calcium carbonate (YDC) to obtain pure cultures. All isolated bacteria were Gram-negative, catalase, and gelatinase positive and were capable of reducing nitrate to nitrite, hydrolyzing starch, and producing hydrosulfuric acid. The isolated facultative anaerobe was positive in D3 media (1), growing at 37°C, but was negative to oxidase, urease, indole, and potato soft rot tests. The isolate also produced acid from different carbon sources like L-arabinose, maltose, trehalose, and palatinose. The identity of two isolates (A1 and A2) was assessed by partial sequencing of the 16S rRNA gene (GenBank Accession Nos. KJ650322.1 and KJ650323.1, respectively). A BlastN search of GenBank revealed 100% nt identity with Pantoea agglomerans (GenBank Accession No. KC764985.1). Pathogenicity was corroborated by spray inoculating plants with a bacterial suspension, prepared after growing a single colony in nutrient broth to a cell density of 108 CFU/ml (48 h) in saline solution. Prior to inoculation, Gram differentiation 3% KOH rapid test (3) and Congo red staining were both performed again to check for the initial description of the pathogen. Inoculation was done by spraying the suspension of each isolate onto 20 21-day-old plants of cultivar Cimarron. Leaves were punctured before inoculation with sterile needles to facilitate bacterial infection. Control plants were sprayed with sterile distilled water. All plants were incubated for 72 h in a humidified chamber (78% humidity) at 31°C and then observed for 15 days for the appearance of disease symptoms. Inoculated plants developed symptoms similar to those observed in the field. Control plants did not show any symptoms. To show virulence consistency, this process was done twice, fulfilling Koch's postulates. These results indicated that the causative agent of this kind of rice leaf blight disease observed in Venezuela is P. agglomerans. A few reports worldwide establish P. agglomerans as a rice pathogen (2,4,5). To our knowledge, this is the first report of rice leaf blight caused by this pathogen in Venezuela, although its impact on rice production has yet to be determined.References:(1) Holt, G., et al. 1994. Beryey's Manual of Determinative Bacteriology. Williams and Wilkins, Baltimore, MD. Google Scholar(2) Azegami, K., et al. 1983. Natl. Inst. Agric. Sci. Ser. C (Jpn.) 37:1. Google Scholar(3) Suslow, T., et al. 1982. Phytopathology 72:917. Crossref, ISI, Google Scholar(4) Yoshida, H., and Yasugi, M. 1980. Ann. Phytopathol. Soc. Jpn. 47:398. Google Scholar(5) Xie, G. 2001. IRRN 26, 1:25. ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 99, No. 4 April 2015SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 17 Apr 2015Published: 1 Apr 2015First Look: 10 Oct 2014Accepted: 30 Sep 2014 Pages: 552-552 Information© 2015 The American Phytopathological SocietyCited byLeaf blight disease of rice caused by Pantoea: profile of an increasingly damaging disease in rice16 November 2022 | Tropical Plant Pathology, Vol. 48, No. 1Beneficial Effect and Potential Risk of Pantoea on Rice Production4 October 2022 | Plants, Vol. 11, No. 19Advanced diagnostic approaches developed for the global menace of rice diseases: a review5 April 2022 | Canadian Journal of Plant Pathology, Vol. 44, No. 5A new bacterial leaf blight disease of oat ( Avena sativa ) caused by Pantoea agglomerans in China18 October 2021 | Plant Pathology, Vol. 71, No. 2Pantoea agglomerans (bacterial grapevine blight)CABI Compendium, Vol. CABI CompendiumAssociations of Pantoea with Rice Plants: As Friends or Foes?16 December 2021 | Agriculture, Vol. 11, No. 12Genomics-Informed Multiplex PCR Scheme for Rapid Identification of Rice-Associated Bacteria of the Genus PantoeaKossi Kini, Raoul Agnimonhan, Rachelle Dossa, Drissa Silué, and Ralf Koebnik14 October 2021 | Plant Disease, Vol. 105, No. 9Characterization, Phylogenetic Analyses, and Pathogenicity of Enterobacter cloacae on Rice Seedlings in Heilongjiang Province, ChinaPeng Cao, Chenxu Li, Kefei Tan, Chuanzeng Liu, Xi Xu, Shaoyong Zhang, Xiangjing Wang, Junwei Zhao, and Wensheng Xiang22 April 2020 | Plant Disease, Vol. 104, No. 6Design of a new multiplex PCR assay for rice pathogenic bacteria detection and its application to infer disease incidence and detect co-infection in rice fields in Burkina Faso27 April 2020 | PLOS ONE, Vol. 15, No. 4Molecular characterization and phylogenetic analysis of Pantoea stewartii subspecies stewartii causing bronzing disease of jackfruit in Malaysia based on cps and hrp gene sequences8 August 2019 | Journal of Plant Pathology, Vol. 102, No. 1An Efficient Inoculation Technique to Assess the Pathogenicity of Pantoea Species Associated to Bacterial Blight of RiceBIO-PROTOCOL, Vol. 10, No. 17Pantoea: a newly identified causative agent for leaf blight disease in rice6 July 2019 | Journal of Plant Diseases and Protection, Vol. 126, No. 6First report of Pantoea agglomerans on Oryza sativa in Turkey10 December 2018 | Journal of Plant Pathology, Vol. 101, No. 2Pantoea agglomerans as a New Etiological Agent of a Bacterial Necrotic Disease of Mango TreesJosé A. 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