Produção Nacional
Revisado por pares
Evaluation of the antimutagenic activity and mode of action of the fructooligosaccharide inulin in the meristematic cells of Allium cepa culture
2014; Research Foundation of Ribeirão Preto; Volume: 13; Issue: 3 Linguagem: Inglês
10.4238/2014.february.14.14
ISSN1676-5680
AutoresM.O. Mauro, João Renato Pesarini, Maria Aparecida Marin‐Morales, Maria Tereza Ferreira Duenhas Monreal, Antônio Carlos Duenhas Monreal, Mário Sérgio Mantovani, Rodrigo Juliano Oliveira,
Tópico(s)Plant Pathogens and Resistance
ResumoThis study evaluated the mutagenicity and antimutagenicity of inulin in a chromosomal aberration assay in cultures of the meristematic cells of Allium cepa. The treatments evaluated were as follows: negative control--seed germination in distilled water; positive control--aqueous solution of methyl methanesulfonate (10 μg/mL MMS); mutagenicity--aqueous solutions of inulin (0.015, 0.15, and 1.50 μg/mL); and antimutagenicity--associations between MMS and the different inulin concentrations. The antimutagenicity protocols established were pre-treatment, simultaneous simple, simultaneous with pre-incubation, and post-treatment. The damage reduction percentage (DR%) was 43.56, 27.77, and 55.92% for the pre-treatment; -31.11, 18.51, and 7.03% for the simultaneous simple; 30.43, 19.12, and 21.11% for the simultaneous with pre-incubation; and 64.07, 42.96, and 53.70% for the post-treatment. The results indicated that the most effective treatment for inhibiting damages caused by MMS was the post-treatment, which was followed by the pre-treatment, suggesting activity by bioantimutagenesis and desmutagenesis. The Allium cepa assay was demonstrated to be a good screening test for this type of activity because it is easy to perform, has a low cost, and shows DR% that is comparable to that reported studies that evaluated the prevention of DNA damage in mammals by inulin.
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