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Analytical applications of affibodies

2014; Elsevier BV; Volume: 65; Linguagem: Inglês

10.1016/j.trac.2014.10.014

1879-3142

Celine I.L. Justino, Armando C. Duarte, Teresa Rocha‐Santos,

Advanced biosensing and bioanalysis techniques

Affibodies are a new class of engineered affinity proteins widely used in imaging, diagnostics and therapeutics, due to their improved properties, such as small size, robustness, high stability, and high imaging contrast compared to the best known affinity molecules (i.e., antibodies). Affibodies can also be used as biological receptors in bioassays and their incorporation in biosensors constitutes a research field of high potential at its very beginning. This review provides an up-to-date overview of the analytical applications of affibodies, and their isolation and characteristics. We also address trends in the future outlook on using affibodies in research.