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Vesicles in rat epididymal fluid. Existence of two populations differing in ultrastructure and enzymatic composition

2009; Wiley; Volume: 27; Issue: 4 Linguagem: Inglês

10.1111/j.1439-0272.1995.tb01099.x

1439-0272

Walter M. Fornés, Miguel A. Sosa, F Bertini, Mario H. Burgos,

Endoplasmic Reticulum Stress and Disease

AndrologiaVolume 27, Issue 4 p. 233-237 Vesicles in rat epididymal fluid. Existence of two populations differing in ultrastructure and enzymatic composition W. M. Fornés, W. M. Fornés Instituto de Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, ArgentinaSearch for more papers by this authorM. A. Sosa, M. A. Sosa Instituto de Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, ArgentinaSearch for more papers by this authorDr. F. Bertini, Corresponding Author Dr. F. Bertini Instituto de Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, ArgentinaInstituto de Histología y Embriología, UNC, Casilla de Correo 56, 5500 Mendoza, Argentina.Search for more papers by this authorM. H. Burgos, M. H. Burgos Instituto de Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, ArgentinaSearch for more papers by this author W. M. Fornés, W. M. Fornés Instituto de Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, ArgentinaSearch for more papers by this authorM. A. Sosa, M. A. Sosa Instituto de Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, ArgentinaSearch for more papers by this authorDr. F. Bertini, Corresponding Author Dr. F. Bertini Instituto de Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, ArgentinaInstituto de Histología y Embriología, UNC, Casilla de Correo 56, 5500 Mendoza, Argentina.Search for more papers by this authorM. H. Burgos, M. H. Burgos Instituto de Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, ArgentinaSearch for more papers by this author First published: July‐August 1995 https://doi.org/10.1111/j.1439-0272.1995.tb01099.xCitations: 20AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Summary Glycosidase activity is very high in rat epididymal fluid as a consequence of the secretory capacity of the epithelium. The mechanism of this secretion is, so far, unknown. Membrane-bound vesicles with activity of β-galactosidase and N-acetyl-β-D-glucosaminidase were previously isolated by us from rat epididymal fluid. We report here the existence of two populations of epididymal vesicles separated by centrifugation in a sucrose gradient. They were found to differ in isopicnic equilibrium, size, ultrastructure, and enzymatic activity. Seven days after castration the protein content and specific activities of both enzymes were found decreased in the fractions containing the vesicles. A role in enzyme secretion by the epididymal epithelium is suggested for each vesicle population. Citing Literature Volume27, Issue4July‐August 1995Pages 233-237 RelatedInformation