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Effects of Chronic Ethanol Ingestion and Folate Deficiency on the Activity of 10-Formyltetrahydrofolate Dehydrogenase in Rat Liver

2005; Wiley; Volume: 29; Issue: 12 Linguagem: Inglês

10.1097/01.alc.0000191756.02856.a8

1530-0277

Hyesun Min, EunSun Im, Jung‐Sook Seo, Ju Ae Mun, Betty J. Burri,

Liver Disease Diagnosis and Treatment

Alcoholism: Clinical and Experimental ResearchVolume 29, Issue 12 p. 2188-2193 Effects of Chronic Ethanol Ingestion and Folate Deficiency on the Activity of 10-Formyltetrahydrofolate Dehydrogenase in Rat Liver Hyesun Min, Hyesun Min Department of Food and Nutrition (HM, JAM), Hannam University, Daejeon, Korea; Department of Food and Nutrition (E-SI, J-SS), Yeungnam University, Gyeongsan, Korea; Western Human Nutrition Research Center (BJB), Agriculture Research Service, United States Department of Agriculture, CASearch for more papers by this authorEun-Sun Im, Eun-Sun Im Department of Food and Nutrition (HM, JAM), Hannam University, Daejeon, Korea; Department of Food and Nutrition (E-SI, J-SS), Yeungnam University, Gyeongsan, Korea; Western Human Nutrition Research Center (BJB), Agriculture Research Service, United States Department of Agriculture, CASearch for more papers by this authorJung-Sook Seo, Corresponding Author Jung-Sook Seo Department of Food and Nutrition (HM, JAM), Hannam University, Daejeon, Korea; Department of Food and Nutrition (E-SI, J-SS), Yeungnam University, Gyeongsan, Korea; Western Human Nutrition Research Center (BJB), Agriculture Research Service, United States Department of Agriculture, CAReprint requests: Jung Sook Seo, Department of Food and Nutrition, Yeungnam University, 214-1 Dae-Dong, Gyeongsan 712-749, Korea; Fax: +82-53-815-2874; E-mail: [email protected]Search for more papers by this authorJu Ae Mun, Ju Ae Mun Department of Food and Nutrition (HM, JAM), Hannam University, Daejeon, Korea; Department of Food and Nutrition (E-SI, J-SS), Yeungnam University, Gyeongsan, Korea; Western Human Nutrition Research Center (BJB), Agriculture Research Service, United States Department of Agriculture, CASearch for more papers by this authorBetty J. Burri, Betty J. Burri Department of Food and Nutrition (HM, JAM), Hannam University, Daejeon, Korea; Department of Food and Nutrition (E-SI, J-SS), Yeungnam University, Gyeongsan, Korea; Western Human Nutrition Research Center (BJB), Agriculture Research Service, United States Department of Agriculture, CASearch for more papers by this author Hyesun Min, Hyesun Min Department of Food and Nutrition (HM, JAM), Hannam University, Daejeon, Korea; Department of Food and Nutrition (E-SI, J-SS), Yeungnam University, Gyeongsan, Korea; Western Human Nutrition Research Center (BJB), Agriculture Research Service, United States Department of Agriculture, CASearch for more papers by this authorEun-Sun Im, Eun-Sun Im Department of Food and Nutrition (HM, JAM), Hannam University, Daejeon, Korea; Department of Food and Nutrition (E-SI, J-SS), Yeungnam University, Gyeongsan, Korea; Western Human Nutrition Research Center (BJB), Agriculture Research Service, United States Department of Agriculture, CASearch for more papers by this authorJung-Sook Seo, Corresponding Author Jung-Sook Seo Department of Food and Nutrition (HM, JAM), Hannam University, Daejeon, Korea; Department of Food and Nutrition (E-SI, J-SS), Yeungnam University, Gyeongsan, Korea; Western Human Nutrition Research Center (BJB), Agriculture Research Service, United States Department of Agriculture, CAReprint requests: Jung Sook Seo, Department of Food and Nutrition, Yeungnam University, 214-1 Dae-Dong, Gyeongsan 712-749, Korea; Fax: +82-53-815-2874; E-mail: [email protected]Search for more papers by this authorJu Ae Mun, Ju Ae Mun Department of Food and Nutrition (HM, JAM), Hannam University, Daejeon, Korea; Department of Food and Nutrition (E-SI, J-SS), Yeungnam University, Gyeongsan, Korea; Western Human Nutrition Research Center (BJB), Agriculture Research Service, United States Department of Agriculture, CASearch for more papers by this authorBetty J. Burri, Betty J. Burri Department of Food and Nutrition (HM, JAM), Hannam University, Daejeon, Korea; Department of Food and Nutrition (E-SI, J-SS), Yeungnam University, Gyeongsan, Korea; Western Human Nutrition Research Center (BJB), Agriculture Research Service, United States Department of Agriculture, CASearch for more papers by this author First published: 03 May 2006 https://doi.org/10.1097/01.alc.0000191756.02856.a8Citations: 20AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Abstract: Background: We recently observed that ethanol feeding impairs 10-formyltetrahydrofolate (10-FTHF) dehydrogenase (EC 1.5.1.6.) and 10-FTHF hydrolase activity in rats. In the present study, we explored the effects of folate deficiency or sufficiency combined with alcoholic intake on 10-FTHF and possible mechanisms by which chronic ethanol ingestion produces folate deficiency. Methods: Sprague-Dawley rats were fed either folate-sufficient (FS) or folate-deficient (FD) diets; with or without ethanol (E) for four weeks. Hepatic 10-FTHF dehydrogenase and hydrolase activity, plasma folate and homocysteine were measured at baseline and after feeding experimental diets. Results: Liver weight increased slightly with either folate deficiency or ethanol consumption. In rats fed the folate-sufficient diet with ethanol (FSE), plasma folate was decreased slightly (p < 0.05) and plasma homocysteine elevated compared to rats fed the FS diet without ethanol. Ethanol did not affect plasma folate and plasma homocysteine in FD rats. Red-blood cell (RBC) folate was increased similarly in rats by ethanol feeding (FSE and FDE > FS and FD). Feeding folate deficient or ethanol (FSE, FD and FDE) diets depressed hepatic activities of 10-FTHF dehydrogenase, which catalyzes the oxidative deformylation of 10-FTHF to tetrahydrofolate (THF) and carbon dioxide. Rats consuming the FDE diet had the lowest enzyme activities of the experimental groups, implying that folate deficiency and ethanol consumption each affect enzyme activity. Conclusions: We confirm that ethanol decreases hepatic 10-FTHF dehydrogenase activity and show that this decrease occurs irrespective of folate status. 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