Comparative cytotoxicity of five root canal sealers on cultured human periodontal ligament fibroblasts
2010; Wiley; Volume: 43; Issue: 3 Linguagem: Inglês
10.1111/j.1365-2591.2009.01676.x
ISSN1365-2591
AutoresMei‐Chi Chang, Li‐Deh Lin, Yi‐Jane Chen, Yi‐Ling Tsai, Ya-Wen Cheng, Chin‐Sung Kuo, Hsiao‐Hua Chang, Tseng‐Fang Tai, Hsueh-Jen Lin, Jiiang‐Huei Jeng,
Tópico(s)Dental Trauma and Treatments
ResumoChang MC, Lin LD, Chen YJ, Tsai YL, Cheng YA, Kuo CS, Chang HH, Tai TF, Lin HJ, Jeng JH. Comparative cytotoxicity of five root canal sealers on cultured human periodontal ligament fibroblasts. International Endodontic Journal , 43 , 251–257, 2010. Abstract Aim To evaluate the cytotoxicity of current root canal sealers to periodontal ligament (PDL) fibroblasts. Methodology Five root canal sealers (Canals, Canals‐N, Topseal, Sealapex, Tubliseal) were prepared and placed into transwells. After initial setting for 1 h, the transwells with sealers were placed into cultured PDL fibroblasts. They were cultured for further 3 or 18 h. Morphological changes were observed. Cell viability was estimated by 3‐(4,5‐dimethyl‐thiazol‐2‐yl)‐2,5‐ diphenyl‐tetrazolium bromide (MTT) assay. Results Marked retraction and death of PDL fibroblasts were observed after exposure to Canals or Topseal for 3 h. A 3‐h exposure of PDL fibroblasts to Tubliseal stimulated MTT reduction. Canals‐N showed little cytotoxicity even after an exposure of 18 h. Conclusion Canals was the most toxic sealer, followed by Topseal. Sealapex and Tubliseal had comparable and moderate cytotoxicity to PDL fibroblasts, whereas Canals‐N showed little cytotoxicity. Exposure to Tubliseal may modulate MTT reduction in PDL fibroblasts. Canals‐N had good biocompatibility.
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