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On-line identification of sugarcane (Saccharum officinarum L.) methoxyflavones by liquid chromatography–UV detection using post-column derivatization and liquid chromatography–mass spectrometry

2005; Elsevier BV; Volume: 1082; Issue: 1 Linguagem: Inglês

10.1016/j.chroma.2005.01.083

1873-3778

Renata Colombo, Janete H. Yariwake, Emerson Ferreira Queiroz, Karine Ndjoko, Kurt Hostettmann,

Phytochemical Studies and Bioactivities

Sugarcane (Saccharum officinarum L., Gramineae) bagasse and leaves were investigated for their flavonoid content and transgenic sugarcane ("Bowman-Birk" and "Kunitz") was compared with non-modified ("control") plants. Analyses were carried out by high-performance liquid chromatography coupled to diode array UV detection (LC/UV), also using post-column addition of shift reagents, and tandem MS (atmospheric pressure chemical ionization–MS/MS and collision-induced dissociation–MS). On-line UV and MS data demonstrated the presence of methoxyflavone glycosides and aglycones in a total of seven compounds. Three naturally occurring flavones glycosides and two unusual erythro- and threo-diastereoisomeric flavolignan 7-O-glucosides were identified together with their aglycones.