Quantification of prostaglandins in human seminal fluid
1982; Elsevier BV; Volume: 24; Issue: 3 Linguagem: Inglês
10.1016/0090-6980(82)90163-0
ISSN1878-416X
AutoresKerstin Svanborg, M. Bygdeman, P. Eneroth, Erik Bendvold,
Tópico(s)Veterinary Equine Medical Research
ResumoA method is described for measurement of the prostaglandins present in human seminal plasma. The PGEs and the 19-hydroxy-PGEs are converted to the respective PGB-compounds. They are determined with UV-absorbance after purification with two chromatography steps. The PGFs and the 19-hydroxy-PGFs are determined by gas chromatography, which also allows measurement of the 8 beta-isomers. Recovery of added PG was in the average 99.2% (range 89.9-107.7). Mean variation between duplicate analyses was 5.6% (range 0.9-9.1). The method has been simplified to allow at least limited clinical use.
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