Identification and Simultaneous Determination of Mangiferin, Neomangiferin, Timosaponin A-III, and Timosaponin C in Rhizoma Anemarrhenae by Rapid Resolution Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry
2010; Taylor & Francis; Volume: 43; Issue: 14 Linguagem: Inglês
10.1080/00032711003717307
ISSN1532-236X
AutoresYanqing Zhang, Junbo Xie, Yan Liu, Dupei Wang,
Tópico(s)Natural Compound Pharmacology Studies
ResumoA new and rapid method was developed for simultaneous determination of mangiferin, neomangiferin, timosaponin A-III, and C in Rhizoma Anemarrhenae using rapid resolution liquid chromatography coupled with triple quadrupole mass spectrometry. The analysis was performed on an Eclipse Plus C18 column (I.D. 4.6 × 100 mm, 3.5 μm). Electrospray ionization–tandem interface in the negative mode was employed prior to mass spectrometric detection. Quantitation was based on multiple reaction monitoring (MRM) for determination. Limits of detection (LODs) ranged from 0.7 to 3 pg. The average recoveries ranged from 98.16 to 100.7% with RSDs ≤ 2.03%. The established method was validated, sensitive, and reliable.
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