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Fluorescence sensing of fermentation parameters using fiber optics

1988; Wiley; Volume: 32; Issue: 1 Linguagem: Inglês

10.1002/bit.260320109

1097-0290

Beth Junker, D. I. C. Wang, T. Alan Hatton,

Advanced Chemical Sensor Technologies

A method has been developed for measuring fermentation parameters such as dissolved oxygen, pH, and cell density that differs from traditional techniques that require electrodes and off-line samples. Fluorescent indicators, each sensitive to a single variable, are dissolved directly into a fermentation broth. A fiber-optic probe fluorimeter measures the fluorescence intensities that can then be correlated with parameter values. In addition, an integrated scatter scanning technique can be used to monitor cell density in situ. Results have been obtained using simulated baker's yeast broth and during actual baker's yeast fermentations.