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Rapid Analysis of Doxorubicin in Plasma by Radial Compression Liquid Chromatography

1985; Elsevier BV; Volume: 74; Issue: 11 Linguagem: Inglês

10.1002/jps.2600741117

1520-6017

Adnan El‐Yazigi, Iman Al‐Saleh,

Cancer Treatment and Pharmacology

A reversed-phase liquid chromatographic method for the analysis of doxorubicin (Adriamycin) in plasma is described. The analysis was performed on a radial compression separation system with a 10-μm phenyl cartridge. The mobile phase consisted of methanol:acetonitrile:0.001 M tetrabutylammonium phosphate (36.4:8.6:55) adjusted to pH 3.5 with 85% phosphoric acid. The flow rate was 2.8 mL/min. Fluorometric detection at excitation and emission wavelengths of 478 and 594 nm, respectively, was used. C18 sample cleanup cartridges were utilized for extraction. A doxorubicin-related compound, 4-demethoxydaunorubicin (IMI 30), was used as an internal standard. The retention times for doxorubicin and IMI 30 were 3.34 and 7.21 min, respectively. The assay was highly linear (r>0.997) in the concentration range utilized in this study (i.e., 10–5,000 ng/mL). No interferences from doxorubicin metabolites or other anticancer drugs were observed. The assay is currently used for monitoring doxorubicin levels in high-risk patients.