Molecular cloning and the complete nucleotide sequence of cDNA to mRNA for S-100 protein of rat brain
1984; Oxford University Press; Volume: 12; Issue: 19 Linguagem: Inglês
10.1093/nar/12.19.7455
ISSN1362-4962
AutoresRyozo Kuwano, Hiroshi Usui, Toshinaga Maeda, Toshikazu Fukui, Nobuko Yamanari, Eiko Ohtsuka, Morio Ikehara, Yasuo Takahashi,
Tópico(s)Antimicrobial Peptides and Activities
ResumoThe complete nucleotide sequence of mRNA for S-subunit of rat brain S- 100 protein was determined from recombinant cDNA clones.The sequence was composed of 1488 bp which included the 276 bp of the complete coding region, the 120 bp of the 5'-noncoding region and the 1092 bp of the 3'-noncoding region containing two polyadenylation signals.In addition, the poly(A) tail was also found.The amino acid sequence deduced from the nucleotide sequence was homologous to the amino acid sequence of bovine S-100 a subunit except 4 residues showing species differences.From the viewpoint of evolutionary implications, the homology between the nucleotide sequence of S-100 and those of rat intestinal Ca-binding protein (ICaBP) and calmodulin (CaM) was examined.A dot-blot hybridization of poly(A) RNA from the developing rat brains using a labeled cDNA showed a rapid increase in S-100 mRNA at 10-20 postna- tal days.The presence of S-100 mRNA in C-6 glioma cells is also described.
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