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The effect of dentine‐bonding agents on substance P release in human dental pulp

2010; Wiley; Volume: 43; Issue: 2 Linguagem: Inglês

10.1111/j.1365-2591.2009.01646.x

1365-2591

Javier Caviedes‐Bucheli, José Antonio Correa-Ortíz, A. C. Ballestero, María Camila Querubín Jiménez, Alejandro García Muñoz, Claudia Rivero, Nelson Lombana, Hugo Roberto Muñoz,

Dental Anxiety and Anesthesia Techniques

Caviedes‐Bucheli J, Correa‐Ortiz JA, Ballestero AC, Jimenez M, Munoz A, Rivero C, Lombana N, Munoz HR. The effect of dentine‐bonding agents on substance P release in human dental pulp. International Endodontic Journal, 43 , 95–101, 2010 . Abstract Aim To quantify the effect of dentine‐bonding agents on Substance P (SP) release in healthy human dental pulp tissue. Methodology Forty pulp samples were obtained from healthy pre‐molars where extraction was indicated for orthodontic reasons. In thirty of these pre‐molars, a standardized Class V cavity preparation was performed, and teeth were divided equally into three groups: (i) Unetched‐cavity control group: Class V cavities only; (ii) Experimental Group I: ‘One‐step’ self‐etch bonding agent was placed in the cavity; and (iii) Experimental Group II: ‘Two‐step’ total‐etch bonding agent was placed in the cavity. The remaining ten healthy pre‐molars where extracted without treatment and served as an intact‐teeth control group. SP was measured by radioimmunoassay. Results Greater SP release was found in the ‘one‐step’ bonding agent group, followed by the ‘two‐step’ bonding agent group and the unetched‐cavity control group. The lower SP values were for the intact‐teeth control group. anova showed statistically significant differences between groups ( P = 0.0001). Tukey HSD post hoc tests showed statistically significant differences in SP release between the intact‐teeth control group and the three other groups ( P < 0.01) and between the unetched‐cavity control group and the ‘one‐step’ bonding agent group ( P < 0.05). No significant difference was found between the ‘two‐step’ bonding agent and the unetched‐cavity control group. Conclusion Dentine‐bonding agents placed over Class V cavity preparations increased SP release. One‐step dentine‐bonding agents increased SP release most.