Artigo Revisado por pares

Automated on-line SPE LC–MS/MS method to quantitate 6beta-hydroxycortisol and cortisol in human urine: Use of the 6beta-hydroxycortisol to cortisol ratio as an indicator of CYP3A4 activity

2005; Elsevier BV; Volume: 821; Issue: 2 Linguagem: Inglês

10.1016/j.jchromb.2005.04.030

ISSN

1873-376X

Autores

Yuchen Barrett, Billy Akinsanya, Simon Chang, O. Vesterqvist,

Tópico(s)

Hormonal Regulation and Hypertension

Resumo

A sensitive method for quantitation of urinary 6beta-hydroxycortisol (6beta-HC) and cortisol using on-line SPE and LC–MS/MS was developed and validated. Human urine samples were injected directly onto an on-line solid phase extraction apparatus, Prospekt-2, followed by HPLC separation and electrospray triple quadrupole LC–MS/MS detection. The inter-day precision for the 6beta-HC:cortisol ratio was 7–9%. The lower limit of quantitation was 1 and 0.2 ng/mL for 6beta-HC and cortisol, respectively. Using the method we observed a diurnal variation on the 6beta-HC:cortisol ratio in healthy volunteers with the maximal ratio observed in the 2–10 pm urine collection period.

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