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Fluorometric Assay Protocol for Protease-Catalyzed Transesterification Reactions in Organic Solvents

2004; American Chemical Society; Volume: 69; Issue: 8 Linguagem: Inglês

10.1021/jo040104i

1520-6904

Min Su Han, Sang Oh Jung, Mahn‐Joo Kim, Dong H. Kim,

Biofuel production and bioconversion

A flourometric assay protocol for a subtilisin-catalyzed transesterification reaction in n-hexane has been developed. The method makes use of a Michael acceptor that forms a fluorescent adduct with thiophenol, one of the products generated in the transesterification reaction. The method may be employed for screening a biocatalyst useful for transesterification reactions in organic solvents and for optimizing the transesterification reaction conditions.