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DNA Optical Detection Based on Porous Silicon Technology: from Biosensors to Biochips

2007; Multidisciplinary Digital Publishing Institute; Volume: 7; Issue: 2 Linguagem: Inglês

10.3390/s7020214

1424-8220

Luca De Stefano, Paolo Arcari, Annalisa Lamberti, Carmen Sanges, Lucia Rotiroti, Ilaria Rea, Ivo Rendina,

Advanced biosensing and bioanalysis techniques

A photochemical functionalization process which passivates the porous silicon surface of optical biosensors has been optimized as a function of the thickness and the porosity of the devices. The surface modification has been characterized by contact angle measurements. Fluorescence measurements have been used to investigate the stability of the DNA single strands bound to the nanostructured material. A dose-response curve for an optical label-free biosensor in the 6-80 μM range has been realized.