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Organization of the human skeletal myosin heavy chain gene cluster.

1992; National Academy of Sciences; Volume: 89; Issue: 24 Linguagem: Inglês

10.1073/pnas.89.24.12078

1091-6490

Sung-Joo Yoon, Stephan Seiler, Raju Kucherlapati, Leslie A. Leinwand,

Neurogenetic and Muscular Disorders Research

Myosin is an important structural and enzymatic component of skeletal muscle. Multiple myosin isoforms are encoded by a multigene family and are expressed in different developmental stages and fiber types. In humans and mice, skeletal myosin heavy chain (MYH) genes are clustered on a single chromosome (17p and 11, respectively). Since the structural organization of the gene cluster may affect its expression as well as shed light on MYH genetic alterations, a physical map of the human MYH gene cluster was constructed. Nine yeast artificial chromosomes containing MYH genes were isolated and used to construct a contiguous set (contig) of overlapping yeast artificial chromosomes. This contig encompasses a genetic marker mapped to 17p13.1. Six MYH genes were located within a 500-kilobase segment of human DNA. The order of the genes within this cluster does not correspond to the developmental pattern of expression of individual members.