Radiation Chemistry of d(TpApCpG) in Oxygenated Solution
1988; Taylor & Francis; Volume: 54; Issue: 3 Linguagem: Inglês
10.1080/09553008814551791
ISSN1362-3095
AutoresChitta R. Paul, John C. Wallace, James L. Alderfer, Harold C. Box,
Tópico(s)Genomics and Chromatin Dynamics
ResumoSummaryThe radiation chemistry of the DNA tetranucleoside triphosphate d(TpApCpG) was investigated. The tetramer was X-irradiated in oxygenated aqueous solution and the various products separated by high-performance liquid chromatography. The principal modifications of the tetramer were analysed intact using nuclear magnetic resonance (NMR) spectroscopy and in some instances also by fast atom bombardment (FAB) mass spectrometry. The principal radiation-induced lesions of d(TpApCpG) were found to be a formamido modification derived from the thymine base, two stereoisomeric forms of a 1-carbamoyl-2-oxo-4,5-dihydroxyimidazolidine modification derived from the cytosine base and an 8-hydroxyguanine modification.
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