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Investigation on the morphological protective effect of 5-hydroxymethylfurfural extracted from wine-processed Fructus corni on human L02 hepatocytes

2010; Elsevier BV; Volume: 130; Issue: 2 Linguagem: Inglês

10.1016/j.jep.2010.05.024

1872-7573

Mingyan Wang, Fengming Zhao, Haiyan Peng, Chenghua Lou, Li Yu, Xia Ding, Xiaoyi Yu, Guangming Yang, Dongqing Xu, Lihua Jiang, Xu Zhang, Lihong Ye, Baochang Cai,

Phytochemistry and Biological Activities

To determine the mode of action of 5-hydroxymethylfurfural (5-HMF) extracted from wine-processed Fructus corni on hepatoprotective activities, the effects of 5-HMF on H2O2-induced human L02 hepatocytes injury was examined. Hepatocytes L02 injured by H2O2 was treated by 5-HMF. The morphological changes of the cells were observed under inverted phase-contrast, fluorescence, and transmission electron microscopy and the activities of caspase-9 and caspase-3 were tested by enzyme-linked immunosorbent detector. It revealed that 5-HMF improved the morphology of H2O2-treated human L02 hepatocytes, and also inhibited the level of caspase-9 and caspase-3 of them. These results suggested a morphological hepatocyte protective effect and the anti-apoptosis mechanism by 5-HMF.