Portal de Periódicos da CAPES

Sunscreen protection against drug-induced phototoxicity in animal models

1979; Elsevier BV; Volume: 49; Issue: 2 Linguagem: Inglês

10.1016/0041-008x(79)90244-8

1096-0333

Frank J. Akin, Arthur P. Rose, Thomas W. Chamness, Edward Marlowe,

Animal testing and alternatives

Abstract Phototoxicity was induced in animal models by photoreactive drugs in order to test sunscreen efficacy. In combination with uv-A irradiation, 8-methoxypsoralen and to a lesser extent chlorpromazine and demeclocycline elicited cutaneous erythema, edema, and ulcerative lesions in mice and guinea pigs. A commercial preparation of 5% para-amino-benzoic acid (PABA) in alcohol with proven effectiveness in preventing normal sunburn failed to protect against phototoxic drug reactions. An experimental, broad-spectrum sunscreen, PL-254—containing esters of PABA and benzophenone-blocked virtually all phototoxic response to all three drugs. Absorbance spectra of the PABA product and of PL-254 in solution and on the skin of hairless mice confirmed that PABA absorbs uv energy primarily in the uv-B range while PL-254 absorbed both uv-A and uv-B radiant energy. The uv absorbance spectra of both products on skin differed significantly from their spectra in solution.