Submicrometer Intracellular Chemical Optical Fiber Sensors
1992; American Association for the Advancement of Science; Volume: 258; Issue: 5083 Linguagem: Inglês
10.1126/science.1439785
ISSN1095-9203
AutoresWeihong Tan, Zhong-You Shi, Steve Smith, Duane Birnbaum, Raoul Kopelman,
Tópico(s)Advanced Fluorescence Microscopy Techniques
ResumoA thousandfold miniaturization of immobilized optical fiber sensors, a millionfold or more sample reduction, and at least a hundredfold shorter response time, all simultaneously, were achieved by combining nanofabricated optical fiber tips with near-field photopolymerization. Specifically, pH optical fiber sensors were prepared with internal calibration, making use of the differences in both fluorescence and absorption of the acidic and basic dye species. The submicrometer sensors have excellent detection limits, as well as photostability, reversibility, and millisecond response times. Successful applications include intracellular and intraembryonic measurements. Potential applications include spatially and temporally resolved chemical analysis and kinetics inside single biological cells and their substructures.
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