Marburg virus, a filovirus: méssenger RNAs, gene order, and regulatory elements of the replication cycle
1992; Elsevier BV; Volume: 24; Issue: 1 Linguagem: Inglês
10.1016/0168-1702(92)90027-7
ISSN1872-7492
AutoresHeinz Feldmann, Elke Mühlberger, Anke Randolf, Christiane Will, Michael P. Kiley, Anthony Sanchez, Hans‐Dieter Klenk,
Tópico(s)Viral Infections and Outbreaks Research
ResumoThe genome of Marburg virus (MBG), a filovirus, is 19.1 kb in length and thus the largest one found with negative-strand RNA viruses. The gene order — 3' untranslated region-NP-VP35-VP40-GP-VP30-VP24-L-5' untranslated region-resembles that of other non-segmented negative-strand (NNS) RNA viruses. Six species of polyadenylated subgenomic RNAs, isolated from MBG-infected cells, are complementary to the negative-strand RNA genome. They can be translated in vitro into the known structural proteins NP, GP (non-glycosylated form), VP40, VP35, VP30 and VP24. At the gene boundaries conserved transcriptional start (3'-NNCUNCNUNUAAUU-5') and stop signals (3'-UAAUUCUU-UUU-5') are located containing the highly conserved pentamer 3'-UAAUU-5'. Comparison with other NNS RNA viruses shows conservation primarily in the termination signals, whereas the start signals are more variable. The intergenic regions vary in length and nucleotide composition. All genes have relatively long 3' and 5' end non-coding regions. The putative 3' and 5' leader RNA sequences of structured and replicated in a basically similar fashion. Conserved sequences in the L proteins of NNS RNA viruses (Tordo et al., 1988; Barik et al., 1990; Stec et al., 1991; Mühlberger et al., 1992), conservation in the leader RNA sequences (Fig. 5D) and the transcription signals (Figs. 6A and 7), and complementarity at the very extremities of the genomes (Mühlberger et al., 1992) are further indications that these viruses employ comparable mechanisms in the transcription and replication and share a common evolutionary lineage.
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