Produção Nacional
Revisado por pares
Sensitivity of PCR and real-time PCR for the diagnosis of human visceral leishmaniasis using peripheral blood
2013; Asian Pacific Journal of Tropical Disease Editorial Office; Volume: 3; Issue: 1 Linguagem: Inglês
10.1016/s2222-1808(13)60003-1
ISSN2222-1808
AutoresLima Júnior, Denielly Christina Rodrigues Zorzenon, Maria Elizabeth Cavalheiros Dorval, Elenir Rose Jardim Cury Pontes, Elisa Teruya Oshiro, Rodrigo Casquero Cunha, Renato Andreotti, Maria de Fátima Cepa Matos,
Tópico(s)T-cell and Retrovirus Studies
ResumoTo evaluate the effectiveness of PCR and real-time PCR for the diagnosis of human visceral leishmaniasis using peripheral blood samples. DNA extraction was performed using Promega Wizard® Genomic kits. PCR employing RV1/RV2 primers yielded 145-bp amplicons. Real-time PCR was performed with the same primers and SYBR Green ROX Plus mix. These techniques were used to analyze 100 peripheral blood samples from patients with clinical signs of the disease. The sensitivity and specificity levels were 91,3%% and 29,6%, respectively, for real-time PCR and 97.78% and 61.82%, respectively, for PCR. Real-time PCR proved to be a satisfactory method for the diagnosis of human visceral leishmaniasis.
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