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A Biotinylated Conducting Polypyrrole for the Spatially Controlled Construction of an Amperometric Biosensor

1999; American Chemical Society; Volume: 71; Issue: 17 Linguagem: Inglês

10.1021/ac9901788

1520-6882

Serge Cosnier, Margarita Stoytcheva, Anne Senillou, Hubert Perrot, Rosa dos Prazeres Melo Furriel, Francisco A. Leone,

Biotin and Related Studies

A new biotin derivative functionalized by an electropolymerizable pyrrole group has been synthesized. The electrooxidation of this biotin pyrrole has allowed the formation of biotinylated conducting polypyrrole films in organic electrolyte. Gravimetric measurements based on a quartz crystal microbalance, modified by the biotinylated polymer, revealed an avidin−biotin-specific binding at the interface of polymer−solution. The estimated mass increase corresponded to the anchoring of 1.5 avidin monolayers on the polypyrrole surface. In addition, the subsequent grafting of biotinylated glucose oxidase was corroborated by electrochemical permeation studies. Enzyme multilayers composed of glucose oxidase or polyphenol oxidase were elaborated on the electrode surface modified by the biotinylated polypyrrole film. The amperometric response of the resulting biosensors to glucose or catechol has been studied at +0.6 or −0.2 V vs SCE, respectively.