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Comparative cytogenetic analysis on four tree frog species (Anura, Hylidae, Hylinae) from Brazil

2003; Karger Publishers; Volume: 103; Issue: 1-2 Linguagem: Inglês

10.1159/000076304

1424-8581

Sanae Kasahara, A.P. Zampieri Silva, Simone Gruber, Célio F. B. Haddad,

Amphibian and Reptile Biology

A comparative cytogenetic analysis was carried out on four Hylinae tree frogs from Brazil (<i>Aparasphenodon brunoi, Corythomantis greeningi, Osteocephalus langsdorffii</i>, and <i>Scinax fuscovarius</i>) using Giemsa staining, BrdU replication banding, Ag-NOR staining, C-banding, DAPI and CMA₃ fluorochrome staining, and fluorescence in situ hybridization (FISH) with an rDNA probe. All the species share closely similar 2n = 24 karyotypes, almost indistinguishable by standard staining. The technique of BrdU incorporation allowed the identification of each pair of homologs and the establishment of extensive homeology for the great majority of the chromosomes, mainly of <i>A. brunoi, C. greeningi,</i> and <i>O. langsdorffii</i>. Despite highly conserved replication banding patterns, the use of the other banding techniques disclosed some minor differences, which reinforces the importance of extensive cytogenetic analyses for the karyotypic characterization of Anuran species. The present cytogenetic data confirm the closer proximity of <i>A. brunoi, C. greeningi,</i> and<i> O. langsdorffii</i>, whereas <i>S. fuscovarius </i>is phylogenetically more distant.