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Orientation-Selected 15 N-HYSCORE Detection of Weakly Coupled Nitrogens around the Archaeal Rieske [2Fe−2S] Center

2004; American Chemical Society; Volume: 126; Issue: 43 Linguagem: Inglês

10.1021/ja045898x

1943-2984

Toshio Iwasaki, Asako Kounosu, Taketoshi Uzawa, Rimma I. Samoilova, Sergei A. Dikanov,

Ammonia Synthesis and Nitrogen Reduction

The weakly coupled 15N atoms around a reduced Rieske [2Fe−2S] cluster of the uniformly 15N-labeled, hyperthermostable archaeal Rieske protein appear to produce readily observable cross-peaks in the HYSCORE spectra, with the well-resolved couplings of 0.3−0.4 MHz for the Nε and 1.1 MHz for the peptide backbone nitrogens, in addition to the contributions from the coordinated Nδ atoms. These features can be used for structure-mechanism studies of the biological redox protein system involving the weakly coupled nitrogens in coupled electron−proton transfer reactions.