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?-Galactosidase immobilization into poly(hydroxyethyl methacrylate) membrane and performance in a continuous system

1999; Wiley; Volume: 72; Issue: 10 Linguagem: Inglês

10.1002/(sici)1097-4628(19990606)72

1097-4628

M. Yakup Arıca, Türker Baran, Adil Deni̇zli̇,

Pancreatic function and diabetes

Journal of Applied Polymer ScienceVolume 72, Issue 10 p. 1367-1373 β-Galactosidase immobilization into poly(hydroxyethyl methacrylate) membrane and performance in a continuous system M. Yakup Arica, Corresponding Author M. Yakup Arica Kırıkkale University, Department of Biology, 71450 Yahşihan-Kırıkkale, TurkeyKırıkkale University, Department of Biology, 71450 Yahşihan-Kırıkkale, Turkey===Search for more papers by this authorTürker Baran, Türker Baran Kırıkkale University, Department of Biology, 71450 Yahşihan-Kırıkkale, TurkeySearch for more papers by this authorAdil Denizli, Adil Denizli Hacettepe University, Department of Chemistry, Ankara, TurkeySearch for more papers by this author M. Yakup Arica, Corresponding Author M. Yakup Arica Kırıkkale University, Department of Biology, 71450 Yahşihan-Kırıkkale, TurkeyKırıkkale University, Department of Biology, 71450 Yahşihan-Kırıkkale, Turkey===Search for more papers by this authorTürker Baran, Türker Baran Kırıkkale University, Department of Biology, 71450 Yahşihan-Kırıkkale, TurkeySearch for more papers by this authorAdil Denizli, Adil Denizli Hacettepe University, Department of Chemistry, Ankara, TurkeySearch for more papers by this author First published: 23 March 1999 https://doi.org/10.1002/(SICI)1097-4628(19990606)72:10 3.0.CO;2-DCitations: 19Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract The activity of β-galactosidase immobilized into a poly(2-hydroxyethyl methacrylate) (pHEMA) membrane increased from 1.5 to 10.8 U/g pHEMA upon increase in enzyme loading. The Km values for the free and the entrapped enzyme were found to be 0.26 and 0.81 mM, respectively. The optimum reaction temperatures for the free and the entrapped β-galactosidase were both found to be 50°C. Similarly, the optimum reaction pH was 7.5 for both the free and the entrapped enzyme. The immobilized β-galactosidase was characterized in a continuous system during lactose hydrolysis and the operational inactivation rate constant (kiop) of the entrapped enzyme was found to be 3.1 × 10−5 min−1. © 1999 John Wiley & Sons, Inc. J Appl Polym Sci 72: 1367–1373, 1999 REFERENCES 1 White, C. A.; Kenedy, J. F. Enzyme Microbiol Technol 1980, 2, 82–89. 10.1016/0141-0229(80)90062-9 CASWeb of Science®Google Scholar 2 Hayashi, T.; Hyon, S. H.; Cha, W. I.; Ikade, Y. Polym J 1993, 25, 489–495. 10.1295/polymj.25.489 CASWeb of Science®Google Scholar 3 Clark, D. S. TIBTECH 1994, 12, 439–446. 10.1016/0167-7799(94)90018-3 CASPubMedWeb of Science®Google Scholar 4 Mohy Elden, M. S.; Bencivenga, U.; Portaccio, M.; Stellato, S.; Rossi, S.; Santucci, M.; Canciglia, P.; Castagnolo, D.; Gaeta, F. S.; Mita, D. C. J Appl Polym Sci 1998, 68, 613–623. 10.1002/(SICI)1097-4628(19980425)68:4 3.0.CO;2-S CASWeb of Science®Google Scholar 5 Arιca, M. Y.; Denizli, A.; Salih, B.; Pişkin, E.; Hasιrcι, V. J Membr Sci 1997, 129, 65–76. 10.1016/S0376-7388(96)00334-1 CASWeb of Science®Google Scholar 6 Arιca, M. Y.; Alaedinoğu, N. G.; Hasιrcι, V. Enzyme Microbiol Technol 1998, 22, 152–157. 10.1016/S0141-0229(97)00139-7 CASWeb of Science®Google Scholar 7 Arιca, M. Y.; Testereci, H. N.; Denizli, A. J Chromatogr A 1998, 799, 83–91. 10.1016/S0021-9673(97)01079-0 CASPubMedWeb of Science®Google Scholar 8 Arιca, M. Y.; Hasιrcι, V.; Alaedinoğlu, N. G. Biomaterials 1995, 16, 767–768. 10.1016/0142-9612(95)99638-3 Google Scholar 9 Piedade, A. P.; Gill, M. H.; Cavaco, M. C.; Andrade, M. E. Polym Int 1995, 38, 269–275. 10.1002/pi.1995.210380309 CASWeb of Science®Google Scholar 10 Siso, M. I. G.; Ramil, A. F.; Belmonte, E. R.; Torres, A. R.; Cerdan, E. Process Biochem 1994, 29, 7–15. 10.1016/0032-9592(94)80053-7 CASWeb of Science®Google Scholar 11 Gekas, V.; Lopes-Leiva, M. Process Biochem 1985, 22, 2–12. Google Scholar 12 Batsalova, K.; Kunchev, K.; Popova, Y.; Kozhukarova, A.; Kirova, N. Appl Microbiol Biotechnol 1987, 26, 227–230. 10.1007/BF00286313 CASWeb of Science®Google Scholar 13 Saito, T.; Yoshida, Y.; Kawashima, K.; Lin, K. H.; Maeda, S.; Kobayashi, T. Appl Microbiol Biotechnol 1994, 40, 618–621. 10.1007/BF00173317 CASWeb of Science®Google Scholar 14 Carrara, C. R.; Rubiolo, C. A. Biotechnol Prog 1994, 10, 220–224. 10.1021/bp00026a012 CASWeb of Science®Google Scholar 15 Park, T. G.; Hoffman, A. S. J Biomed Mater Res 1990, 24, 21–38. 10.1002/jbm.820240104 CASPubMedWeb of Science®Google Scholar 16 Bodalo, A.; Gomez, E.; Gomez, J. L.; Bastida, J.; Maxima, M. F.; Diaz, F. Process Biochem 1991, 26, 349–355. 10.1016/0032-9592(91)85025-J CASWeb of Science®Google Scholar 17 Rejikumar, S.; Devi, S. J Appl Polym Sci 1995, 55, 879–878. 10.1002/app.1995.070550605 CASWeb of Science®Google Scholar 18 Lowry, O. H.; Rosebrough, N.; Farr, A.; Randall, R. J Biol Chem 1951, 193, 263–275. 10.1016/S0021-9258(19)52451-6 Google Scholar 19 Craven, G. R.; Steers, E.; Anfinsen, C. J. J Biol Chem 1965, 240, 2468–2472. 10.1016/S0021-9258(18)97348-5 CASPubMedWeb of Science®Google Scholar 20 Gacesa, P.; Hubble, J. Enzyme Technology; Alden: Oxford, 1987, pp 45–64. Google Scholar 21 Park, T. G.; Hoffman, A. S. Enzyme Microbiol Technol 1993, 15, 476–485. 10.1016/0141-0229(93)90079-H CASPubMedWeb of Science®Google Scholar 22 Khare, S. K.; Gupta, M. G. Biotechnol Bioeng 1988, 31, 829–833. 10.1002/bit.260310810 CASPubMedWeb of Science®Google Scholar Citing Literature Volume72, Issue106 June 1999Pages 1367-1373 ReferencesRelatedInformation