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High-performance thin-layer chromatographic method for monitoring degradation products of rifampicin in drug excipient interaction studies

1994; Elsevier BV; Volume: 685; Issue: 1 Linguagem: Inglês

10.1016/0021-9673(94)00730-6

1873-3778

K.C. Jindal, R. S. Chaudhary, S.S. Gangwal, Anil Kumar Singla, S. Khanna,

Antibiotics Pharmacokinetics and Efficacy

A thin-layer Chromatographic assay for the determination of rifampicin and its degradation components in drug-excipient interaction studies is described. The chromatography was performed on thin-layer plates (silica gel) with a mobile phase consisting of chloroform-methanol-water (80:20:2.5, v/v/v). The peaks were quantified by densitometric evaluation of the chromatograms. The method shows a limit of detection of 10 ng per band and good precision and linearity in the range 50–3000 ng per band for rifampicin, 3-formylrifamycin SV, rifampicin N-oxide and 25-desacetylrifampicin and 100–350 ng per band for rifampicin quinone