Voltar
Artigo Acesso aberto Revisado por pares
BIBTEX RIS

Varicella-Zoster Virus ORF47 Protein Serine Kinase: Characterization of a Cloned, Biologically Active Phosphotransferase and Two Viral Substrates, ORF62 and ORF63

2001; American Society for Microbiology; Volume: 75; Issue: 18 Linguagem: Inglês

10.1128/jvi.75.18.8854-8858.2001

ISSN

1098-5514

Autores

T. K. Kenyon, Jennifer M. Lynch, J. Hay, William T. Ruyechan, Charles Grose,

Tópico(s)

Food Allergy and Anaphylaxis Research

Resumo

ABSTRACT Varicella-zoster virus (VZV) codes for a protein serine kinase called ORF47; the herpes simplex virus (HSV) homolog is UL13. No recombinant alphaherpesvirus serine kinase has been biologically active in vitro. We discovered that preservation of the intrinsic kinase activity of recombinant VZV ORF47 required unusually stringent in vitro conditions, including physiological concentrations of polyamines. In this assay, ORF47 phosphorylated two VZV regulatory proteins: the ORF62 protein (homolog of HSV ICP4) and the ORF63 protein (homolog of HSV ICP22). Of interest, ORF47 kinase also coprecipitated ORF63 protein from the kinase assay supernatant.

Referência(s)