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Effects of chloroform, diethyl ether and a propiophenone derivative, 3-dimethylamino-2-methyl-2-phenoxypropiophenone hydrochloride, upon cyclic 3′,5′-nucleotide phosphodiesterase

1971; Elsevier BV; Volume: 20; Issue: 8 Linguagem: Inglês

10.1016/0006-2952(71)90396-0

1873-2968

Issaku Ueda, F Okumura,

Cholinesterase and Neurodegenerative Diseases

The effects of chloroform, diethyl ether and 3-dimethylamino-2-methyl-2-phenoxypropiophenone HC1 (U-0882) were investigated upon crude cyclic 3′,5′-nucleotide phosphodiesterase prepared from dog heart. Chloroform noncompetitively inhibited the enzyme at a partial pressure close to the clinical application. The partial pressure which gave a half-maximal inhibition was estimated to be 30 mb. Diethyl ether, on the other hand, inhibited the enzyme preparation when its partial pressure was above the clinical application and a half-maximal inhibition was induced at 425 mb. The enzyme preparation was inhibited by U-0882 competitively. The concentration which inhibited the enzyme half maximally was about 0.9 mM. A possible role of cyclic AMP in mediating the arrhythmogenic effect of catecholamines is discussed.