Phagocytosis in Acanthamoeba : II. Soluble and insoluble mannose‐rich ligands stimulate phosphoinositide metabolism
1990; Wiley; Volume: 145; Issue: 3 Linguagem: Inglês
10.1002/jcp.1041450318
ISSN1097-4652
AutoresPhilip G. Allen, Eliezar A. Dawidowicz,
Tópico(s)Ion channel regulation and function
ResumoAbstract The generation of second messengers during phagocytosis of yeast by Acan‐thamoeba castellanii was examined. The kinetics of binding and internalization of yeast by Acanthamoeba were measured and this was compared with the generation of known second messengers. We observed stimulated degradation of PI‐4, 5‐P 2 to 1,4,5 IP 3 with kinetics similar to that observed for the binding of yeast to amoeba. Similar production of IP 3 could be induced upon treatment with a soluble mannosylated glycoprotein. We propose that the Acanthamoeba man‐nose receptor stimulates the degradation of PI‐4, 5‐P 2 to 1,4,5 IP 3 as an initial event in phagocytosis.
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