Characterization of zeta (ζ): a new opioid receptor involved in growth
1989; Elsevier BV; Volume: 482; Issue: 2 Linguagem: Inglês
10.1016/0006-8993(89)91192-x
ISSN1872-6240
AutoresIan S. Zagon, Steven R. Goodman, Patricia J. McLaughlin,
Tópico(s)Pharmacological Receptor Mechanisms and Effects
ResumoEndogenous opioid systems (i.e., opioids and opioid receptors) are known to play a role in neural cancer. Using [3H]-[Met5]enkephalin, a potent ligand involved in growth, specific and saturable binding was detected in homogenates of S20Y neuroblastoma transplanted into A/Jax mice; the data fit a single binding site. Scatchard analysis yielded aKd of 0.49 nM and a binding capacity of 5.32 fmol/mg protein. Binding was dependent on protein concentration, time, temperature, and pH, and was sensitive to Na+ and guanine nucleotides. Optimal binding required protease inhibitors, and pretreatment of the tumor homogenates with trypsin markedly reduced [3H]-[Met5]enkephalin binding, suggesting that the binding site was proteinaceous in character. Displacement experiments indicated that [Met5]enkephalin was the most potent displacer of [3H]-[Met5]enkephalin; other ligands selective for μ, δ, κ, ɛ, and σ were not highly competitive. Given the functional significance of [Met5]enkephalin as a potent regulator of normal and abnormal growth, and that the receptor recognized by [Met5]enkephalin does not resemble any previously described, the present study has demonstrated the presence of a new opioid receptor termed zeta (ζ) (from the Greek 'Zoe', life) related to the proliferation of cells and tissues.
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