Human ribosomal protein L37a: cloning of the cDNA and analysis of differential gene expression in tissues and cell lines
1993; Elsevier BV; Volume: 132; Issue: 2 Linguagem: Inglês
10.1016/0378-1119(93)90209-l
ISSN1879-0038
AutoresDeba P. Saha, Padma S. Tirumalai, Louis A. Scala, Richard Howells,
Tópico(s)Glycosylation and Glycoproteins Research
ResumoA cDNA corresponding to human ribosomal protein L37a (hL37a) was obtained by screening a SHSY5Y neuroblastoma library. The full-length cDNA contained 366 nucleotides (nt) in addition to a poly(A) tail. The pyrimidinerich sequence, CTTTCT, that is common to many ribosomal protein-encoding cDNAs was present at the 5' terminus. The nt sequence displayed 85% identity with rat L37a (rL37a) cDNA. The predicted protein contains 92 amino acids with a Mr of 10 277, is highly basic, and has 100% sequence identity with rL37a. A putative zinc-finger domain is present in the central region of the protein. Human lymphocytes and several human cell lines express hL37a mRNA at significantly higher levels than the rat cell lines and rat tissues tested. The hL37a gene does not contain introns.
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