A compact acousto-optic lens for 2D and 3D femtosecond based 2-photon microscopy
2010; Optica Publishing Group; Volume: 18; Issue: 13 Linguagem: Inglês
10.1364/oe.18.013720
ISSN1094-4087
AutoresPaul A. Kirkby, K. M. Naga Srinivas Nadella, R. Angus Silver,
Tópico(s)Optical Coherence Tomography Applications
ResumoWe describe a high speed 3D Acousto-Optic Lens Microscope (AOLM) for femtosecond 2-photon imaging. By optimizing the design of the 4 AO Deflectors (AODs) and by deriving new control algorithms, we have developed a compact spherical AOL with a low temporal dispersion that enables 2-photon imaging at 10-fold lower power than previously reported. We show that the AOLM can perform high speed 2D raster-scan imaging (>150 Hz) without scan rate dependent astigmatism. It can deflect and focus a laser beam in a 3D random access sequence at 30 kHz and has an extended focusing range (>137 mum; 40X 0.8NA objective). These features are likely to make the AOLM a useful tool for studying fast physiological processes distributed in 3D space.
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