A novel platform for enhanced biosensing based on the synergy effects of electrospun polymer nanofibers and graphene oxides
2012; Royal Society of Chemistry; Volume: 138; Issue: 5 Linguagem: Inglês
10.1039/c2an36663k
ISSN1364-5528
AutoresXiaofang Su, Jun Ren, Xianwei Meng, Xiangling Ren, Fangqiong Tang,
Tópico(s)Conducting polymers and applications
ResumoA novel biosensing platform was developed by combining the advantages of electrospun poly(vinyl alcohol) (PVA)/chitosan nanofibers and graphene oxides (GO). Glucose oxidase (GOD) was employed as a model enzyme. By co-electrospinning the solution of PVA, chitosan, GOD and GO, the PVA/chitosan/GOD/GO nanofibers were directly modified on the platinum (Pt) electrode. The UV-vis spectra and the FTIR spectra were used to characterize the GO nanosheets. The morphologies of fabricated electrospun nanofibers were characterized by high resolution scanning electron microscopy. After a thin layer of nafion was modified on the surface of matrix, the as-prepared electrode was used to detect glucose. The electrode exhibited great advantages in high sensitivity, low detection limit and wide linear range. In the meantime, the electrode showed good stability, acceptable reproducibility, and excellent anti-interference capability for ascorbic acid, uric acid, lactose and sucrose. Moreover, the novel biosensor was successfully applied for the glucose determination in human serum samples. The mechanism of efficient biosensing of the nafion/PVA/chitosan/GOD/GO/Pt electrode was analyzed in detail and the results show that it can be due to the synergy effects of electrospun nanofibers and GO nanosheets.
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