Determination of tryptophan by high-performance liquid chromatography of alkaline hydrolysates with spectrophotometric detection
2003; Elsevier BV; Volume: 85; Issue: 2 Linguagem: Inglês
10.1016/j.foodchem.2003.07.026
ISSN1873-7072
AutoresMaría del Mar Yust, Justo Pedroche, Julio Girón‐Calle, Javier Vioque, Francisco Millán, Manuel Alaiz,
Tópico(s)Enzyme Structure and Function
ResumoA procedure for quantitation of tryptophan in feedstuff is described. It consist of hydrolysis in sodium hydroxide at 100 °C for 4 h, neutralization of the resulting hydrolysate to pH 7, dilution with sodium borate buffer (pH 9), and analysis by reverse-phase high-performance liquid chromatography with spectrophotometric determination of tryptophan at 280 nm. The recovery of tryptophan from lysozyme, added to some feedstuff before hydrolysis, ranges from 98.6 to 100%. A reduction of the time of analysis has been achieved as compared to previous methods for analysis of tryptophan.
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